FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309058
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60190.45979628098984154No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47660.36407859697584066No Hit
GCCTTGGGCTGACCTAGGACGGTCAATCTGGTCCCTCCGCCGAAGACCATAGTGCTGATACTTGTATATGAGCAG39460.30143813337529735No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38860.29685468481916005No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT38510.2941810064947466No Hit
GACCTAGGACGGTCAATCTGGTCCCTCCGCCGAAGACCATAGTGCTGATACTTGTATATGAGCAGCAATAATAAT35430.2706526372399084No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCACCATCTCCTG32720.2499507279280215No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32640.2493396014538699No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATCTGGTCCCTC32340.24704787717580123No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32210.2460547966553048No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30880.23589481902253376No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28930.22099861121508751No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27050.2066371390725239No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26580.20304677103688298No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26250.20052587433100746No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25970.19838693167147672No Hit
CCATAGTGCTGATACTTGTATATGAGCAGCAATAATAATCAGCCTCGTCC25750.19670633386755973No Hit
GCACTATGGTCTTCGGCGGAGGGACCAGATTGACCGTCCTAGGTCAGCCC25090.19166454045580866No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAATATGTCTCCTGGTACCAACAACACGCAGGCAAAGC25020.191129804790926No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24870.18998394265189167No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAATATGTCTCCTGGTACCAACAACA24240.18517132166794747No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23830.18203929848792033No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23410.1788308844986242No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23400.17875449368935523No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23240.17753224074105195No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22680.17325435542199047No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21990.16798338958243256No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21340.16301798697995046No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCAC21300.16271242374287465No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20080.15339274501206213No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19650.15010794021349702No Hit
GACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCACCATCT19420.14835095160031106No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19270.14720508946127672No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT17580.13429504269482329No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17290.1320797092260236No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17260.13185053679821673No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17100.13062828384991346No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17050.13024632980356865No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16930.1293296400923412No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAT16520.12619761691231407No Hit
GATTATTATTGCTGCTCATATACAAGTATCAGCACTATGGTCTTCGGCGGAGGGACCAGATTGACCGTCCTAGGT16440.1255864904381624No Hit
TTTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCTGCTCATAT16220.12390589263424538No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15250.11649598413515673No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15080.1151973403775845No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15080.1151973403775845No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15030.11481538633123971No Hit
GGCAAAGCCCCCAAACTCATGGTTTATGAATCCATTAATCGGCCCTCAGG14910.11389869662001226No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14890.11374591500147434No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCAC14860.11351674257366748No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14380.10984998372875762No Hit
CCCCTGAGGGCCGATTAATGGATTCATAAACCATGAGTTTGGGGGCTTTGCCTGCGTGTTGTTGGTACCAGGAGA13990.10687074216726837No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13920.10633600650238569No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC13740.10496097193554449No Hit
GTGCTGATACTTGTATATGAGCAGCAATAATAATCAGCCTCGTCCTCAGC13490.10305120170382061No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATCTGGTC13320.10175255794624836No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13300.10159977632771046No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAAATGGTCAGGGAGG13300.10159977632771046No Hit
ACCTAGGACGGTCAATCTGGTCCCTCCGCCGAAGACCATAGTGCTGATAC13260.10129421309063465No Hit
ATCTGGTCCCTCCGCCGAAGACCATAGTGCTGATACTTGTATATGAGCAGCAATAATAATCAGCCTCGTCCTCAG13140.10037752337940717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5400.056.23649270
CGCGTAT800.051.6067939
GCGTATG1250.049.64826240
GGTATCA13100.048.1959651
ATCGCGT652.2555469E-1047.50092337
CGACGTC8050.045.71681630
CGTATGC3400.045.7053141
ACTCGAC8300.045.46161727
TCGACGT8300.045.1245729
ACGAGAC8350.045.1220622
GACTCGA8450.044.63923326
CCCACGA8250.044.42960419
CTCGACG8500.044.41912528
GACGTCA8100.044.2013631
TCGTATG3250.043.4952740
AGACTCG8750.043.4705125
CACGAGA8550.043.2725821
TCGCGTA803.6379788E-1142.94246738
ACGTCAT6600.042.93213732
TATACAC9150.041.9156883
Produced by FastQC (version 0.11.5)