FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309058
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68810.525645158579681No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC59490.4544489243410147No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42670.32595958315063195No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40580.3099939040134203No Hit
GCCTTGGGCTGACCTAGGACGGTCAATCTGGTCCCTCCGCCGAAGACCATAGTGCTGATACTTGTATATGAGCAG38580.29471574215962926No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT34460.2632427287408197No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCACCATCTCCTG34340.2623260390295923No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34060.26018709637006154No Hit
GACCTAGGACGGTCAATCTGGTCCCTCCGCCGAAGACCATAGTGCTGATACTTGTATATGAGCAGCAATAATAAT32430.2477353944592218No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30650.23413783040934777No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29530.2255820597712248No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28920.22092222040581855No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATCTGGTCCCTC28680.2190888409833636No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28390.2168735075145639No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27590.21076224277304748No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25870.19762302357878717No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAATATGTCTCCTGGTACCAACAACACGCAGGCAAAGC25230.19273401178557406No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCAC24340.18593522976063703No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24140.18440741357525794No Hit
CCATAGTGCTGATACTTGTATATGAGCAGCAATAATAATCAGCCTCGTCC24100.1841018503381821No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAATATGTCTCCTGGTACCAACAACA23720.1811989995859618No Hit
GCACTATGGTCTTCGGCGGAGGGACCAGATTGACCGTCCTAGGTCAGCCC23720.1811989995859618No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23390.1786781028800863No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22700.17340713704052838No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22130.16905286091219793No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21580.16485136640240539No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21180.16179573403164718No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21000.16042069946480597No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20920.15980957299065435No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20220.1544622163418275No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19390.14812177917250421No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18460.14101743391049135No Hit
GACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCACCATCT17940.13704511182850568No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17830.13620481292654718No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17670.1349825599782439No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17360.1326144448909063No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17010.12994076656649287No Hit
GATTATTATTGCTGCTCATATACAAGTATCAGCACTATGGTCTTCGGCGGAGGGACCAGATTGACCGTCCTAGGT16840.12864212280892062No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16790.12826016876257584No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT16590.12673235257719673No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC16510.1261212261030451No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAT16190.12367672020643851No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15500.1184057543668806No Hit
TTTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCTGCTCATAT15450.11802380032053585No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15300.11687793818150151No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15220.11626681170734986No Hit
GTGCTGATACTTGTATATGAGCAGCAATAATAATCAGCCTCGTCCTCAGC14980.11443343228489496No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14660.11198892638828836No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGCTCTCCTGGACAGTCGATCAC14570.11130140910486777No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14460.11046111020290927No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14210.10855133997118539No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14090.10763465025995793No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAATCTGGTC13220.10098864985355882No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13120.10022474176086926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG200.00788719350.85099413
GGTATCA13550.041.575911
GTGGTAT4600.039.839041
CGCGGAT350.001415052139.0403933
GTATCAA36150.038.9594421
TGGTATC4750.038.563272
TACGTAT800.002221402735.6120270
TTAACGG701.0407293E-634.20774535
TATCAAC41200.034.1683962
ATCAACG42050.033.551973
TCAACGC42250.033.3039634
CAACGCA42950.032.7636765
AACGCAG43550.032.5458686
AACGGCC851.280041E-732.2589437
TAACGGC751.7717775E-631.95555936
ACCCGGT652.4717976E-531.55569834
ACGCAGA46150.030.7134727
GAGTCGT604.0436172E-430.22737164
CCTAACG450.005021587530.152352
CTAACGC450.005026282330.146593
Produced by FastQC (version 0.11.5)