FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718466
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGGACTACACTTTTGGCCAGGGGACCAAGCTGG29670.41296317431861773No Hit
GAGTATTACCAGCGACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC26050.36257804823053563No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24590.3422569752778837No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCGGCTGATGGTGAGAGTGAACT23020.3204048625822238No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21810.30356342540913556No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA19850.2762830808973563No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18890.26292127950383176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15860.22074809385551997No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGACACCAGTGGCCCTGGT15530.2161549746264959No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAGTTCA14980.2084997759114558No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14230.19806086857276475No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCTCCCTGTCTGTGTCTCCAGG13960.19430286193083596No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATT13540.18845707382116897No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13280.18483825261042278No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13280.18483825261042278No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12880.17927083536312088No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGC12740.1773222393265652No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12730.17718305389538266No Hit
GAGTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGG12620.17565201415237464No Hit
CTCTTGCAGGGCCAGTCAGAGTATTACCAGCGACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT12090.16827518629969965No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCGGCTGATGGTGAGAGTGA12080.1681360008685171No Hit
GTTTATTACTGTCAACAGTATAATAACTGGCCTCCGGACTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12050.16771844457496946No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11820.16451717965777088No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11730.16326451077712795No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11510.1602024312911119No Hit
GTCTCCAGCCTCCCTGTCTGTGTCTCCAGGGGAAAGCGCCACCCTCTCTTGCAGGGCCAGTCAGAGTATTACCAG11380.1583930206857388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11190.15574849749327038No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11170.1554701266309053No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGACACCA11120.15477419947499255No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11040.15366071602553216No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10970.15268641800725435No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10950.15240804714488923No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10860.15115537826424633No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGTCCGGAGG10420.14503121929221424No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGTCCGGAGGCCAGTTATTATA9850.13709764971480906No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC9140.1272154841008482No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8920.12415340461483215No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8680.12081295426645101No Hit
GTGTAGTCCGGAGGCCAGTTATTATACTGTTGACAGTAATAAACTGCAAA8640.12025621254172084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8540.11886435822989537No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8410.11705494762452225No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8310.11566309331269677No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8280.11524553701914914No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7830.10898219261593452No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7590.10564174226755337No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7430.10341477536863261No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7420.10327558993745006No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAGTTCACT7250.10090943760734676No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7220.10049188131379912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002391493968.7341639
CCGTCAT502.3852492E-456.01635770
CCTAACG252.8689235E-453.9691242
CGTTTCG200.006349668453.714657
GATACGT200.00779456751.0007933
CCGCTAG200.00804398950.596051
TCGTATG3100.047.7551540
ATCTCGT2800.046.4302837
TGCGTGA551.1463635E-745.31480455
CTAACGC307.051359E-444.9742663
ACGAGAC4300.043.9436522
CGTATGC3100.043.41460441
CGAGACA4500.041.9935223
GGTATCA8000.041.7417451
ATGCCGT3200.041.2675244
CACGAGA4650.040.630421
TATACAC4900.040.6145133
AGGCAAT4050.040.2489132
CTTATAC4900.039.9261321
GTATGCC3700.039.23670242
Produced by FastQC (version 0.11.5)