FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718466
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34860.48520041310235973No Hit
GTATAATAACTGGCCTCCGGACTACACTTTTGGCCAGGGGACCAAGCTGG28380.39500825369606907No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25720.3579849290015116No Hit
GAGTATTACCAGCGACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC24910.3467109090757252No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCGGCTGATGGTGAGAGTGAACT20590.2865828028048648No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA19690.27405611399843555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17170.23898138534043364No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16940.23578012042323507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16190.22534121308454402No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14480.20154050435232845No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14300.1990351665910426No Hit
GAGTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGG14020.19513797451793127No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATT13840.1926326367566454No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGACACCAGTGGCCCTGGT13330.1855341797663355No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAGTTCA13180.1834463982985973No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCTCCCTGTCTGTGTCTCCAGG13100.1823329148491369No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11440.15922813327283405No Hit
CTCTTGCAGGGCCAGTCAGAGTATTACCAGCGACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT11420.15894976241046896No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11370.15825383525455622No Hit
GTCTCCAGCCTCCCTGTCTGTGTCTCCAGGGGAAAGCGCCACCCTCTCTTGCAGGGCCAGTCAGAGTATTACCAG11150.1551917557685402No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11100.15449582861262745No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11000.15310397430080197No Hit
GTTTATTACTGTCAACAGTATAATAACTGGCCTCCGGACTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA10990.15296478886961945No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCGGCTGATGGTGAGAGTGA10980.1528256034384369No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGC10890.15157293455779397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10720.14920678222769065No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10700.14892841136532556No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGACACCA10420.14503121929221424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10360.14419610670511895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10260.14280425239329347No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10250.14266506696211095No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10190.14182995437501564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10050.13988135833846No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGTCCGGAGG9730.13542742454061849No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9660.13445312652234065No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGTCCGGAGGCCAGTTATTATA9460.13166941789868972No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9400.13083430531159443No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9330.1298600072933166No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8410.11705494762452225No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8410.11705494762452225No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8250.11482798072560149No Hit
GTGTAGTCCGGAGGCCAGTTATTATACTGTTGACAGTAATAAACTGCAAA7960.11079160322130761No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC7940.11051323235894252No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7790.10842545089120431No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7780.10828626546002178No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7650.10647685485464864No Hit
CTGCTACTCTGGCTCCCAGATTCCACTGGAGAAATAGTGATGACGCAGTC7390.10285803364390243No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7250.10090943760734676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)