FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306602
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA38550.29504011167899635No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34730.26580397091080527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32350.24758878373062337No Hit
GTCTATTACTGTACGAGAGATCGATATTTTGACTGGTTTGGCGCTGATGC31420.24047108453836746No Hit
ATATTACTGTCAGCACTATGGTGGCTCACTTCTCCCTTTCGGCGGAGGGA31220.23894039654003288No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27690.21192375336942698No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25720.19684647658583104No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCG25590.19585152938691353No Hit
GTATATTACTGTCAGCACTATGGTGGCTCACTTCTCCCTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA25410.19447391018841237No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24050.18406523179973702No Hit
GAGTATTAGCAGCCTATACTTAGCCTGGTACCAACAGAAGCCTGGCCAGA22450.17181972781306012No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22420.17159012461330994No Hit
CCATAGTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTACT20860.15965075822629998No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20220.1547525566316292No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCT19450.14885940783804097No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19100.1461807038409554No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19020.14556842864162153No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGACTGGAGCCTGAAGATT18960.14510922224212117No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG18700.1431193278442862No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18470.14135903664620136No Hit
GCCTATACTTAGCCTGGTACCAACAGAAGCCTGGCCAGACTCCCAGGCTCCTCATCCATGCTACATCTAGGAGGG17950.13737924785053138No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG17350.13278718385552754No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17260.13209837425627696No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCCTATACTTAGCCTGGTACC17220.13179223665661005No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17210.13171570225669332No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17120.13102689265744275No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16850.128960463859691No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16840.12888392945977428No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16790.12850125746019064No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC16670.12758284466118985No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16460.12597562226293854No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15790.12084781746851757No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGC15640.11969980146976661No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15510.11870485427084912No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15480.11847525107109892No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15370.1176333726720149No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15290.11702109747268104No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15210.1164088222733472No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGGG15080.11541387507442971No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15030.11503120307484606No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14990.11472506547517912No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14830.11350051507651145No Hit
GCACTATGGTGGCTCACTTCTCCCTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT14820.1134239806765947No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14480.11082181107942587No Hit
CTCCTGTGCAGCCTCTGGACTCAATTTCAGTAGTTATAGTGTGAACTGGGTCCGCCAGGCCCCAGGGAAGGGG14390.1101330014801753No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14330.1096737950806749No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAAACATCAAAAGCATCAGCGC14300.10944419188092472No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14030.10737776308317298No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13610.1041633182866703No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC13410.1026326302883357No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC13370.10232649268866877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG350.00453043358.49777270
CGTCATT252.5835336E-455.12963540
CCTAACG501.2314558E-954.593742
ATACGAA200.007490698251.5187837
CTAACGC552.8649083E-949.6306723
GGTATCA12250.044.2928661
CGAGACG857.4578566E-1140.15167223
TCTGGAG15850.038.7524770
TCGTATG557.4601157E-637.58838740
GTATCAA32150.036.1947941
TACGTAT951.4678606E-435.9196870
GGGACAA12400.033.5733270
TATCAAC36150.032.0917552
ATCAACG36950.031.396943
CGTATGC553.259457E-431.36001641
TCAATCA1103.4717098E-431.02154570
TCAACGC37600.030.7634264
AATTGCG450.00475653130.48854335
TCGGCAG900.00487590530.33217670
CAACGCA38600.030.0548445
Produced by FastQC (version 0.11.5)