FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306602
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37520.2871570684875731No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA32120.2458284925325386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30820.23587902054336363No Hit
GTCTATTACTGTACGAGAGATCGATATTTTGACTGGTTTGGCGCTGATGC29950.22922052775060806No Hit
ATATTACTGTCAGCACTATGGTGGCTCACTTCTCCCTTTCGGCGGAGGGA27900.2135309757676783No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26630.20381110697825353No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26390.20197428138025197No Hit
GTATATTACTGTCAGCACTATGGTGGCTCACTTCTCCCTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA26360.2017446781805018No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCG25560.19562192618716331No Hit
GAGTATTAGCAGCCTATACTTAGCCTGGTACCAACAGAAGCCTGGCCAGA22470.1719727966128936No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22360.17113091821380957No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20850.15957422382638325No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20620.15781393262829846No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGACTGGAGCCTGAAGATT20200.15459948783179575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19890.1522269214343771No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG19160.14663991024045578No Hit
CCATAGTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTACT18760.14357853424378655No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18470.14135903664620136No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17980.13760885105028156No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17910.13707311025086447No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCCTATACTTAGCCTGGTACC17870.13676697265119753No Hit
GCCTATACTTAGCCTGGTACCAACAGAAGCCTGGCCAGACTCCCAGGCTCCTCATCCATGCTACATCTAGGAGGG17780.13607816305194698No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCT17460.13362906225461157No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17340.1327106494556108No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17320.13255758065577736No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG17240.1319453054564435No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16800.12857779186010734No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16800.12857779186010734No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGC16330.12498067506402101No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15990.1223785054668522No Hit
CTCCTGTGCAGCCTCTGGACTCAATTTCAGTAGTTATAGTGTGAACTGGGTCCGCCAGGCCCCAGGGAAGGGG15830.1211539550681845No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGGG15410.11793951027168181No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15210.1164088222733472No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15170.11610268467368028No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15000.11480159987509585No Hit
GCACTATGGTGGCTCACTTCTCCCTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT14690.1124290334776772No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14590.11166368947850991No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14320.10959726068075817No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14260.1091380542812578No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14080.10776043508275665No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13990.10707162548350609No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC13930.10661241908400569No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13890.10630628148433877No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13830.10584707508483839No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13810.10569400628500493No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13750.10523479988550455No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC13640.1043929214864205No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13560.10378064628708665No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13240.10133154548975128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA250.00639241453.6254369
TTAGACG200.00773368151.1044584
GGTATCA12600.042.7353321
CTAACGC405.3881784E-542.585423
GTATCAA34550.039.258671
GTAGTTT2401.8189894E-1235.24738770
TCTGGAG13250.035.1143870
GGGACAA12050.035.1011370
TGGTATC3800.034.9635352
TATCAAC39150.034.2845732
GTGGTAT3900.034.0800741
TAGTACG502.0040394E-434.069644
AATGGTA7350.033.56894370
ATCAACG40450.033.352443
TCAACGC40550.033.1874434
CAACGCA41400.032.5085455
AACGCAG41800.032.3617256
GTCGGTT652.5290845E-531.45010412
ACGCAGA44150.030.7163627
AGAGTAC44600.030.48167611
Produced by FastQC (version 0.11.5)