FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675859
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGCTCACCTACTTTTGGCCAGGGGACCAAGCTGGTGATCAGACGAACTGTGG27360.404818164735544No Hit
CAGTAATACACTGCAAAATCTTCAGGGTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21350.3158942915608137No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19990.2957717512084621No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18770.27772064883355846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15920.2355520900069393No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13820.20448052034521993No Hit
GTGTATTACTGTCAGCAATATGGTAGCTCACCTACTTTTGGCCAGGGGAC13770.20374072106755994No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA13760.20359276121202796No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12570.18598553840372029No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12360.18287838143754837No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11800.17459262952775653No Hit
GTCAATATGTTAGCAGCAGTAAGTTAGCCTGGTACCAGCAGAAACCTGGC11560.1710415929949886No Hit
GTAATACACTGCAAAATCTTCAGGGTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11560.1710415929949886No Hit
CTCCTGCAGGGCCAGTCAATATGTTAGCAGCAGTAAGTTAGCCTGGTACC11220.1660109579069007No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10950.1620160418075368No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10850.1605364432522168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10480.15506192859753293No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10360.15328641033114895No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGACCCTGAAGATT10200.150919052642637No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT9810.1451486182768891No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9740.14411289928816512No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9580.1417455415996532No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCACCAGCTTGGTCC9450.13982206347773724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8970.13271999041220137No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8900.13168427142347738No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA8870.13124039185688138No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8350.12354647936921755No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8270.12236280052496157No Hit
GACTTCACTCTCTCCATCAGCAGACTGGACCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAGCTCA8170.12088320196964158No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAATATGTTAGCAG7390.10934233323814584No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7370.10904641352708185No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7350.10875049381601784No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG7230.10697497554963387No Hit
AAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGTCTCCCAAACTCCTCATCTATGGCGCATCCAACAGGGCCA7140.1056433368498459No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7070.10460761786112192No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6890.10194434046154598No Hit
GTGCAGCCACAGTTCGTCTGATCACCAGCTTGGTCCCCTGGCCAAAAGTA6820.100908621472822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCA150.002495893767.99711614
GTGCGCA306.781595E-445.3314111
GGTATCA6750.042.3187071
GTATCAA20100.038.743271
CGTATGC901.2369128E-1038.3176241
TCGTATG952.237357E-1036.24921840
TGTTAGA5550.035.80426870
ACGCGTA1250.035.3951627
TATCAAC22700.034.303182
CGCGTAG1300.034.0413728
AGACGCG1300.034.01869225
GACGCGT1300.034.01869226
TCAACGC23100.033.5619854
ATCAACG23300.033.27393
CGAGACG1450.032.8383423
GCGTAGT1350.032.8000229
CAACGCA23550.032.6318935
AACGCAG23850.032.5065776
AATGGTA5600.031.93612770
CGTAGTA1400.031.65204630
Produced by FastQC (version 0.11.5)