FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675859
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGCTCACCTACTTTTGGCCAGGGGACCAAGCTGGTGATCAGACGAACTGTGG27880.4125120772232078No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23020.3406035874346572No Hit
CAGTAATACACTGCAAAATCTTCAGGGTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19950.2951799117863341No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14380.21276627225501177No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14070.20817951673351986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13790.20403664077862393No Hit
GTGTATTACTGTCAGCAATATGGTAGCTCACCTACTTTTGGCCAGGGGAC13680.202409082367772No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA13170.19486312973564013No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12610.1865773778258483No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11920.1763681477941405No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11860.1754803886609485No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11490.1700058740062646No Hit
GTAATACACTGCAAAATCTTCAGGGTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11360.16808239588434865No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10990.16260788122966477No Hit
CTCCTGCAGGGCCAGTCAATATGTTAGCAGCAGTAAGTTAGCCTGGTACC10930.1617201220964728No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGACCCTGAAGATT10310.15254661105348896No Hit
GTCAATATGTTAGCAGCAGTAAGTTAGCCTGGTACCAGCAGAAACCTGGC10200.150919052642637No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10070.14899557452072104No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9520.1408577824664612No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT9460.13997002333326922No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9430.13952614376667324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9210.1362710269449693No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9040.13375570940092535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8720.12902099402390144No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8680.12842915460177345No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8660.12813323489070944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8460.12517403778006952No Hit
GACTTCACTCTCTCCATCAGCAGACTGGACCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAGCTCA8430.12473015821347352No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCACCAGCTTGGTCC8280.12251076038049355No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8240.12191892095836558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8080.11955156326985363No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7850.11614848659261769No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7710.11407704861516973No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7640.11304132962644574No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAATATGTTAGCAG7400.10949029309367782No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG7360.10889845367154984No Hit
AAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGTCTCCCAAACTCCTCATCTATGGCGCATCCAACAGGGCCA6940.10268413973920595No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAAT6940.10268413973920595No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT6790.100464741906226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGC150.002167670870.4580253
CGGTCAT250.006091154654.2800169
ATTCGCG200.00682327652.74620452
GGTATCA9100.041.017361
AATGGTA4100.039.7698270
GTGGTAT2500.036.647151
GTATCAA21750.034.322571
ACGGGTT502.0501355E-433.9350616
ACGTACC1350.032.64677413
TATTAAC450.00502454430.1466334
TATCAAC24800.029.6733972
CGTACCA1501.8189894E-1229.3886214
TCAACGC25000.029.3025284
AACGCAG25250.029.1510376
ATCAACG25100.029.0442183
GACTCGG1650.028.7805947
CAACGCA25500.028.7322225
ATACGTA1551.8189894E-1228.44480711
CAACCTT1000.006770082328.35761370
GGACTCG1800.028.2666536
Produced by FastQC (version 0.11.5)