FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059468
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40130.37877500783412055No Hit
GTATATCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA39200.3699970173709824No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33960.3205382323958817No Hit
GTGATATACCATACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAAT31060.2931660040699672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27900.26333971389414307No Hit
GTATGGTATATCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT27130.25607191533864165No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26340.24861534279468564No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23690.2236027893244534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22130.20887841822499592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21830.20604680839817718No Hit
CTCCTGCAGGGCCAGTCAGAATGTTAACAGCGATTACTTAGCCTGGTACC21710.2049141644674497No Hit
ATACCATACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAATCTGTC21450.20246010261754013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20970.19792952689463011No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA20930.19755197891772094No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20070.1894346974141739No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19690.18584799163353682No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAACAG18280.17253942544748874No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18060.17046291157448834No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17560.16574356186312378No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17270.1630063390305323No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17170.1620624690882594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16750.15809821533071314No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16390.15470028353853066No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16160.15252938267130295No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15910.15016970781562067No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15640.1476212589714838No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGTAATCGCTGTTAACATTCTGA15410.1454503581042561No Hit
GTATTTCTGTCAGCAGTATGGTATATCACCTCGGACGTTCGGCCAAGGGA15100.14252436128321005No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14690.13865449451989112No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14600.1378050115718455No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14230.13431269278543573No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14020.1323305659066626No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC13890.1311035349817078No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13300.1255347023222976No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC12960.12232554451856971No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12860.12138167457629678No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12380.1168510988533868No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12250.115624067928432No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11850.11184858815934036No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11800.11137665318820389No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11660.1100552352690218No Hit
CAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA11600.10948891330365806No Hit
GTGTATTTCTGTCAGCAGTATGGTATATCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA11500.10854504336138514No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT11280.10646852948838473No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTT11150.10524149856342994No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11060.10439201561538432No Hit
GATTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTTCTGTCAGCAGTATGGTATATCA10900.10288182370774768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)