FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156543
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACACACTGGCCTCACACTTTTGGCCAGGGGACGAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT70320.6080188976977078No Hit
GTGTGTATGCTGCATGCAGAAGTAAACCCCAACATCCTCAGCCTCCACCC54260.46915678880940875No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54190.46855153677813965No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTG51070.44157458909871916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45160.3904740247444323No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36520.31576863117065257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35810.30962964628206646No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28820.24919090773105712No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28690.2480668682444146No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27920.24140909590045506No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25600.22134931429268087No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24620.21287578585491418No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23650.20448872199304308No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23140.20007902862236854No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23110.19981963489468182No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGAATTCACACTGAA22270.19255661051945325No Hit
GTGTATGCTGCATGCAGAAGTAAACCCCAACATCCTCAGCCTCCACCCTACTGATTTTCAGTGTGAATTCAGTGC21640.1871093422380318No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21260.18382368835399981No Hit
CTTTTGGCCAGGGGACGAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21180.18313197174683518No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21120.18261318429146173No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20480.1770794514341447No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTCGTCCCCTGGCCAAAAGTG19960.17258329348757462No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19490.1685194584204824No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT18790.16246693810779192No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18770.16229400895600077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18640.16116996946935824No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18260.15788431558532628No Hit
GTCTGGGACCCCAGAGTCCCGGTTAAAAACCCTGTTGATTAGGCGCCTAGGAGATTGGCCTGGCCTCTGGTGAAA18250.1577978510094307No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTCGTCC18130.15676027609868376No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTGTC18030.15589563033972798No Hit
CAGCATACACACTGGCCTCACACTTTTGGCCAGGGGACGAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17980.15546330746025008No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC17190.14863260596449937No Hit
GGCCAAAAGTGTGAGGCCAGTGTGTATGCTGCATGCAGAAGTAAACCCCA17020.14716270817427454No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA16570.1432718022589735No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC16460.14232069192412217No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16360.14145604616516635No Hit
GTGATGGAAACACCCACTTGCATTGGTTTCACCAGAGGCCAGGCCAATCT16270.14067786498210616No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16270.14067786498210616No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16050.13877564431240344No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15580.13471180924531123No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15450.13358776975866873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15360.1328095885756085No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14270.12338494980299046No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT14130.12217444574045236No Hit
GGTCTAGTCAAAGCCTCGTGTCCAGTGATGGAAACACCCACTTGCATTGGTTTCACCAGAGGCCAGGCCAATCTC14040.12139626455739216No Hit
GGTTTCACCAGAGGCCAGGCCAATCTCCTAGGCGCCTAATCAACAGGGTT13650.11802414609746459No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13520.11690010661082206No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13250.11456556306164145No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13210.11421970475805915No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13180.11396031103037242No Hit
GGCCAATCTCCTAGGCGCCTAATCAACAGGGTTTTTAACCGGGACTCTGG13170.11387384645447683No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13080.11309566527141664No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13040.11274980696783432No Hit
AAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12610.10903183020432444No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12450.10764839698999519No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12410.10730253868641286No Hit
GTTTTTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC12340.10669728665514383No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12290.10626496377566592No Hit
GCAGTGGGTCAGGCACTGAATTCACACTGAAAATCAGTAGGGTGGAGGCT12140.10496799513723225No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12120.10479506598544108No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTC12110.10470860140954551No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11960.10341163277111183No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11840.1023740578603649No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11820.10220112870857373No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA11800.10202819955678259No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11730.10142294752551353No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11630.10055830176655775No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11600.10029890803887101No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11580.10012597888707986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)