FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156543
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACACACTGGCCTCACACTTTTGGCCAGGGGACGAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT68460.5919364865811302No Hit
GTGTGTATGCTGCATGCAGAAGTAAACCCCAACATCCTCAGCCTCCACCC61110.52838502329788No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52910.45748407106350564No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTG44980.38891766237831193No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38510.33297508177387264No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36300.3138664105009498No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33990.29389309346907116No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30420.26302523987434967No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27120.2344919298288088No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25660.22186810174805432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24100.2083796279083441No Hit
GTGTATGCTGCATGCAGAAGTAAACCCCAACATCCTCAGCCTCCACCCTACTGATTTTCAGTGTGAATTCAGTGC23170.2003384223500553No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22540.19489115406863386No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22120.19125964188101957No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21460.18555297987191138No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGAATTCACACTGAA21060.18209439683608825No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20960.18122975107713246No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC20800.1798463178628032No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20760.1795004595592209No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20740.1793275304074297No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTCGTCCCCTGGCCAAAAGTG20710.179068136679743No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20390.1763012702510845No Hit
CTTTTGGCCAGGGGACGAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19760.17085400196966302No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19230.1662713794471974No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18840.1628992609872698No Hit
GTCTGGGACCCCAGAGTCCCGGTTAAAAACCCTGTTGATTAGGCGCCTAGGAGATTGGCCTGGCCTCTGGTGAAA18770.16229400895600077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18680.16151582777294057No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTGTC18330.1584895676165953No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17930.15503098458077216No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17850.15433926797360756No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC17000.1469897790224834No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTCGTCC16990.1469033144465878No Hit
CAGCATACACACTGGCCTCACACTTTTGGCCAGGGGACGAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16900.1461251332635276No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16460.14232069192412217No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16110.1392944317677769No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16000.13834332143292555No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT15950.13791099855344763No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15300.13229080112023506No Hit
GGCCAAAAGTGTGAGGCCAGTGTGTATGCTGCATGCAGAAGTAAACCCCA15250.13185847824075714No Hit
GTGATGGAAACACCCACTTGCATTGGTTTCACCAGAGGCCAGGCCAATCT15200.13142615536127927No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15090.1304750450264279No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC14790.12788110774956055No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA14700.12710292656650032No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14360.12416313098605065No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA14130.12217444574045236No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13920.12035868964664523No Hit
GGTCTAGTCAAAGCCTCGTGTCCAGTGATGGAAACACCCACTTGCATTGGTTTCACCAGAGGCCAGGCCAATCTC13670.11819707524925575No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13570.11733242949029997No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13180.11396031103037242No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13170.11387384645447683No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13160.11378738187858126No Hit
GTTTTTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC12980.11223101951246084No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12660.10946415308380232No Hit
GGTTTCACCAGAGGCCAGGCCAATCTCCTAGGCGCCTAATCAACAGGGTT12450.10764839698999519No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12220.1056597117443969No Hit
GGCCAATCTCCTAGGCGCCTAATCAACAGGGTTTTTAACCGGGACTCTGG11980.103584561922903No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT11950.10332516819521627No Hit
GCAGTGGGTCAGGCACTGAATTCACACTGAAAATCAGTAGGGTGGAGGCT11700.1011635537978268No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11680.10099062464603563No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11680.10099062464603563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2450.045.3938371
GGTATCA10850.042.2433361
ATAAACG504.536627E-640.4120523
GTATCAA28550.038.1281281
TGGTATC3250.035.2386362
ACTTGCA25850.033.67803270
TATCAAC32400.033.4760672
ATCAACG33400.032.3659363
ACGCAAT552.774015E-432.2336353
AATGGTA4400.032.17210870
TCAACGC33850.031.8320484
AACGCAG34650.031.5871016
CAACGCA34150.031.5565055
ACGCAGA36400.029.975977
CGCAGAG36950.029.4386378
AGAGTAC36800.029.28282511
GCAGAGT37900.028.700739
CAGAGTA37850.028.56069410
CACGACT500.00866916326.96235517
ACAAACG1455.8935257E-1026.57293148
Produced by FastQC (version 0.11.5)