FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957891
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38850.40557850527878436No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36940.3856388670527231No Hit
GCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACACCCATCTGCTGAAACCTGCATATGAGCAG35540.3710234254210552No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30660.3200781717335271No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCTC27750.2896989323419888No Hit
GACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACACCCATCTGCTGAAA27110.2830175875960835No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26550.27717141094341635No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25200.26307794937002227No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGATGTT24160.2522207641579261No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG23290.24313831114396106No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23040.2405284108525918No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22780.23781411454956775No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21930.2289404535589122No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21540.22486900910437618No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGATGTTGGGAGTTTTAAGCTTGTCTCCTGGTACCAACAGTA20980.219022832451709No Hit
CTCCTGCACTGGAACCAACAGTGATGTTGGGAGTTTTAAGCTTGTCTCCTGGTACCAACAGTACCCCGGCAAAGC20960.21881404042839947No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20800.21714370424192314No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19850.20722608313471994No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19020.19856121416737393No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18980.19814363012075487No Hit
GTAATAGACAGCCGTGTCGTCCTTTCTCAGGGTGTTGATCTCCAAATACA18950.19783044208579056No Hit
GTACCAACAGTACCCCGGCAAAGCCCCCAAACTCATGATTTTTGAAGTCA18570.19386339364290928No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT18440.19250624549139725No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18420.19229745346808771No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18330.19135788936319478No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC17810.1859292967571467No Hit
TCTCCAGCCTGAGGACGAGGCTAATTATTACTGCTGCTCATATGCAGGTT17340.18102268420937248No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17180.17935234802289615No Hit
GTAATAATTAGCCTCGTCCTCAGGCTGGAGACGAGAGATTGTCAGGGAGG17130.17883036796462226No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16990.1773688238014555No Hit
AATTATTACTGCTGCTCATATGCAGGTTTCAGCAGATGGGTGTTCGGCGGAGGGACCAAAGTGACCGTCCTAGGT16840.17580288362663393No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16590.17319298333526464No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16060.1676599947175618No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15680.1636929462746805No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14710.15356653314416777No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACACCCAT14370.15001706874790555No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14190.14813794053811968No Hit
GTTTTAAGCTTGTCTCCTGGTACCAACAGTACCCCGGCAAAGCCCCCAAA13850.1445884761418575No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13830.14437968411854793No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTC13720.14323132799034546No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC13510.14103901174559527No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCC13370.13957746758242848No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13260.13842911145422598No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13110.13686317127940445No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12700.13258293480155886No Hit
GCTCTAGTCTCCCGTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12670.13226974676659453No Hit
GGCTGGAGACGAGAGATTGTCAGGGAGGCCGTGTTGCCCGTCTTGGAGCC12630.13185216271997544No Hit
CTTTGGTCCCTCCGCCGAACACCCATCTGCTGAAACCTGCATATGAGCAG12530.13080820260342774No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCG12410.1295554504635705No Hit
CTGTTGGTACCAGGAGACAAGCTTAAAACTCCCAACATCACTGTTGGTTCCAGTGCAGGAGATGGTGATCGA12210.12746753023047508No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12170.12704994618385598No Hit
GTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12110.12642357011392735No Hit
GTCTATTACTGTGCGAAAGGGGTCGAGCGGGAGTGGGAGCCGCCTACCCT12090.12621477809061782No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGA12050.12579719404399875No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12010.12537960999737965No Hit
CAACAGTACCCCGGCAAAGCCCCCAAACTCATGATTTTTGAAGTCACTAA11740.12256091768270085No Hit
ACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACACCCATCTGCTGAAAC11700.12214333363608178No Hit
GTACTGTTGGTACCAGGAGACAAGCTTAAAACTCCCAACATCACTGTTGGTTCCAGTGCAGGAGATGGTGATCGA11390.11890705727478387No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11290.11786309715823616No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11240.11734111709996231No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.11671474103003368No Hit
CACTAAGAGGCCCTCAGGGATTTCTGATCGTTTCTCTGGCTCCAAGACGGGCAACACGGCCTCCCTGACAATCT11170.11661034501837893No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11140.11629715698341461No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC11050.11535759287852167No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC10920.11400044472700964No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10680.11149494044729515No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG10660.1112861484239856No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10580.11045098033074743No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10550.11013779229578312No Hit
GGAACCAACAGTGATGTTGGGAGTTTTAAGCTTGTCTCCTGGTACCAACA10370.10825866408599727No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10260.10711030795779479No Hit
TAATTAGCCTCGTCCTCAGGCTGGAGACGAGAGATTGTCAGGGAGGCCGTGTTGCCCGTCTTGGAGCCAGAGAAA10260.10711030795779479No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10170.10617074385290184No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10170.10617074385290184No Hit
CCCATCTGCTGAAACCTGCATATGAGCAGCAGTAATAATTAGCCTCGTCC10070.10512678373635415No Hit
GCTGAAACCTGCATATGAGCAGCAGTAATAATTAGCCTCGTCCTCAGGCTGGAGACGAGAGATTGTCAGGGAGGC9910.10345644754987782No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9880.10314325951491349No Hit
ACAGTACCCCGGCAAAGCCCCCAAACTCATGATTTTTGAAGTCACTAAGAGGCCCTCAGGGATTTCTGATCGTTT9870.10303886350325872No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT9860.10293446749160395No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9830.10262127945663965No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9730.10157731934009194No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT9630.10053335922354423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT300.002288487369.49792570
CCGCGAC200.00767157151.20775228
TGCGCGA200.007711292251.14090325
GTGGTAT2850.047.866141
GGTATCA10700.046.854011
TGGTATC3000.045.463332
CGTATGC1850.037.27286541
CGAGACG2400.036.92738323
CGTGTTC4150.036.35515252
TCGTATG1900.036.2479840
ATCTCGT1900.036.1431437
GCGTGTT4200.035.845351
ATGCCGT2050.035.4662644
GTATCAA32400.034.841451
ACGAGAC2550.034.75518822
TATCAAC31650.034.6900332
TCTCGTA1900.034.36670738
CTACATC2300.034.2154333
TAAGATC400.002750584434.0957154
CACGAGA2650.033.4419221
Produced by FastQC (version 0.11.5)