FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957891
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39740.41486975031605894No Hit
GCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACACCCATCTGCTGAAACCTGCATATGAGCAG35280.3683091291180312No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34790.3631937245469474No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33400.3486826789269343No Hit
GACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACACCCATCTGCTGAAA29500.3079682343815737No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27560.2877154081205482No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25240.26349553341664134No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCTC25030.26130321717189114No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG24330.2539954963560572No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23640.24679217155187802No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23520.2455394194120208No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22570.23562179830481758No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22390.2337426700950317No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGATGTT22320.2330118980134483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22290.23269870997848396No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC21820.22779209743070974No Hit
CTCCTGCACTGGAACCAACAGTGATGTTGGGAGTTTTAAGCTTGTCTCCTGGTACCAACAGTACCCCGGCAAAGC20560.21463819996220868No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGATGTTGGGAGTTTTAAGCTTGTCTCCTGGTACCAACAGTA20280.21171511163587506No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19760.20628651902982698No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19040.1987700061906835No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18640.19459416572449265No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18590.1940721856662188No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT18270.19073151329326615No Hit
AATTATTACTGCTGCTCATATGCAGGTTTCAGCAGATGGGTGTTCGGCGGAGGGACCAAAGTGACCGTCCTAGGT17440.18206664432592018No Hit
GTACCAACAGTACCCCGGCAAAGCCCCCAAACTCATGATTTTTGAAGTCA17400.18164906027930108No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17380.18144026825599155No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17110.17862157594131273No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17040.17789080385972933No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17030.17778640784807456No Hit
GTAATAGACAGCCGTGTCGTCCTTTCTCAGGGTGTTGATCTCCAAATACA16610.1734017753585742No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16350.17068747905555015No Hit
GTAATAATTAGCCTCGTCCTCAGGCTGGAGACGAGAGATTGTCAGGGAGG16030.1673468066825975No Hit
TCTCCAGCCTGAGGACGAGGCTAATTATTACTGCTGCTCATATGCAGGTT15540.16223140211151374No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15440.161187441994966No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15230.15899512575021582No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14810.15461049326071546No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14640.15283576106258437No Hit
GGCTGGAGACGAGAGATTGTCAGGGAGGCCGTGTTGCCCGTCTTGGAGCC14160.14782475250315535No Hit
GTTTTAAGCTTGTCTCCTGGTACCAACAGTACCCCGGCAAAGCCCCCAAA13900.14511045620013133No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13690.14291813995538116No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC13580.14176978382717867No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13510.14103901174559527No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACACCCAT13230.1381159234192617No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCG13200.13780273538429738No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13130.13707196330271398No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTC13110.13686317127940445No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13000.13571481515120196No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGA12900.13467085503465426No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC12830.13394008295307086No Hit
GCTCTAGTCTCCCGTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12320.12861588635867754No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCC12250.12788511427709418No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12240.1277807182654394No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12220.12757192624212985No Hit
GTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12110.12642357011392735No Hit
CTGTTGGTACCAGGAGACAAGCTTAAAACTCCCAACATCACTGTTGGTTCCAGTGCAGGAGATGGTGATCGA11760.1227697097060104No Hit
CTTTGGTCCCTCCGCCGAACACCCATCTGCTGAAACCTGCATATGAGCAG11720.1223521256593913No Hit
GTCTATTACTGTGCGAAAGGGGTCGAGCGGGAGTGGGAGCCGCCTACCCT11700.12214333363608178No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11120.11608836496010506No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT11020.11504440484355735No Hit
CAACAGTACCCCGGCAAAGCCCCCAAACTCATGATTTTTGAAGTCACTAA10800.1127476925871524No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10790.11264329657549764No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10660.1112861484239856No Hit
GTACTGTTGGTACCAGGAGACAAGCTTAAAACTCCCAACATCACTGTTGGTTCCAGTGCAGGAGATGGTGATCGA10630.11097296038902131No Hit
TAATTAGCCTCGTCCTCAGGCTGGAGACGAGAGATTGTCAGGGAGGCCGTGTTGCCCGTCTTGGAGCCAGAGAAA10580.11045098033074743No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG10560.1102421883074379No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC10420.10878064414427112No Hit
CACTAAGAGGCCCTCAGGGATTTCTGATCGTTTCTCTGGCTCCAAGACGGGCAACACGGCCTCCCTGACAATCT10350.10804987206268772No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10280.10731909998110434No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10160.10606634784124708No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT10100.10543997177131846No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10050.1049179917130446No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG9900.10335205153822304No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9820.10251688344498487No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9720.10147292332843716No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9710.10136852731678239No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCTCCGCCGAACAC9660.10084654725850853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.049.1940421
GTGGTAT2100.047.0316621
TTAGGCT306.74279E-445.3861734
CGAAACC407.497029E-444.41827469
TGGTATC2550.041.3815082
GTATCAA32950.037.3132821
TATCAAC33850.035.5983122
CCGAAAC551.6268309E-435.29844768
CGTGTCG4700.034.76024613
ATCAACG34400.034.629733
TCAACGC34700.034.1377264
CAACGCA35000.033.8451165
AACGCAG35200.033.751286
GCGTGTT4600.032.7094651
GTAATAG5000.032.6951181
ACAGCCG4800.032.6230168
CGTGTTC4300.032.6202952
GTCGTCC5050.032.354516
TGTCGTC5050.032.354515
GTGTCGT5050.032.3494314
Produced by FastQC (version 0.11.5)