FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542276
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13900.25632703641688No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12280.226452950158222No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGAAACA12260.22608413427848548No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11460.21133149908902477No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG10870.2004514306367975No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGACCACCATCTCCTG10340.19067780982377977No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10230.18864932248522892No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGGTTGTATATGAGCAGCAGTAATAAT10160.1873584669061511No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC9650.1779536619728699No Hit
GATTATTACTGCTGCTCATATACAACCAGCACCACTTATGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGT9550.17610958257418732No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9540.17592517463431906No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC9250.17057734437813954No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC9040.1667047776409061No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGG9000.16596714588143308No Hit
CTCCTGGACAGTCGACCACCATCTCCTGCACTGGAACCAGCAGTGACGTT8890.16393865854288223No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC8710.16061931562525356No Hit
GTATATTACTGTACAAGAGCCGGGAGCTACCGGGCTGACTACTGGGGCCA8650.159512867986044No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8570.15803760446709794No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG8280.15268977421091842No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA7970.14697312807500237No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG7820.1442070089769785No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGC7780.14346937721750547No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT7700.1419941136985594No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGGTTGTATATGAGCAG7690.14180970575869115No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG7160.13203608494567343No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6970.1285323340881765No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT6810.12558180705028435No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6480.1194963450346318No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6480.1194963450346318No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGTGGTG6390.11783667357581748No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGACCAC6190.1141485147784523No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6080.11212002743990145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6060.11175121156016493No Hit
GTCGACCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTATCAACAACA5940.10953831628174582No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5880.10843186864253626No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGACCACCATCT5860.10806305276279975No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC5750.10603456542424891No Hit
GTATTAATACGTGAGACCCACACCAGCCCCTTCCCTGGAGCTTGGCGGAC5670.10455930190530283No Hit
GCTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAAGTCAATAAT5660.10437489396543458No Hit
TATTAATACTGATGGGACTAGCACAAGCTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACGC5630.1038216701458298No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5550.10234640662688371No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG5440.10031791928833288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)