FastQCFastQC Report
Wed 8 May 2024
EGAF00004692858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542276
Total Bases40.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGAAACA15280.2817753321186997No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC13990.2579867078756943No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC13570.2502415744012274No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13480.24858190294241309No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13130.24212762504702404No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12200.22497768663927595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11850.2185234087438869No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11690.21557288170599476No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT11650.2148352499465217No Hit
CTCCTGGACAGTCGACCACCATCTCCTGCACTGGAACCAGCAGTGACGTT11420.21059386732955174No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11170.20598366883284527No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10770.19860735123811488No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGTGGTGCTG10730.19786971947864185No Hit
GATTATTACTGCTGCTCATATACAACCAGCACCACTTATGTCTTCGGAAC10650.19639445595969582No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA10520.19399715274140844No Hit
GTATATTACTGTACAAGAGCCGGGAGCTACCGGGCTGACTACTGGGGCCA10060.18551438750746854No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGTGGTGCTGG9400.17334346347616345No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC9250.17057734437813954No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG9160.16891767291932522No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG9110.16799563321998393No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATA8950.16504510618209178No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT8840.16301661884354093No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCT8740.16117253944485835No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8640.15932846004617576No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7980.14715753601487067No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT7910.14586668043579284No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7910.14586668043579284No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7600.14015003429987682No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7490.13812154696132595No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7130.13148286112606863No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC6980.12871674202804476No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6910.12742588644896694No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG6880.12687266262936217No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTC6700.1235533197117335No Hit
GTCGACCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATA6680.12318450383199699No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT6660.12281568795226047No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC6660.12281568795226047No Hit
GCTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAA6530.12041838473397311No Hit
GTATTAATACGTGAGACCCACACCAGCCCCTTCCCTGGAGCTTGGCGGAC6510.1200495688542366No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG6450.11894312121502704No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG6310.11636141005687141No Hit
TATTAATACTGATGGGACTAGCACAAGCTACGCGGACTCCGTGAAGGGCC6120.11285765919937449No Hit
TATACAACCAGCACCACTTATGTCTTCGGAACTGGGACCACGGTCACCGT6100.11248884331963797No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT5870.10824746070266801No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC5870.10824746070266801No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT5840.10769423688306323No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC5800.10695660512359022No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT5790.10677219718372194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.10566574954451238No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGACCACCATCT5710.10529693366477587No Hit
GGTTGTATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC5700.10511252572490762No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT5700.10511252572490762No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT5580.1028996304464885No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC5490.10123995898767418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)