FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514868
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54210.35785296144614576No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47320.312370450758746No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC44520.2938869921339681No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC42310.27929826229083987No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38670.2552697660786286No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36660.24200128328012738No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTATGCTGCATC36440.2405490115310377No Hit
GATCTATGCTGCATCCAATTTGCGAAGTGGGGCCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA34440.22734654108476776No Hit
GGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT33250.21949107116923716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32910.2172466511933713No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT32800.21652051531882646No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32190.21249376183271415No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA31710.20932516892560935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30040.19830110610297402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29740.19632073553603352No Hit
GCATAGATCAGGAGCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGC28700.18945545090397314No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28650.1891253891428164No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27980.184702561543316No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26990.17816733867241238No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26390.1742065975385314No Hit
CACTATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTACTATTGTCAAC25460.1680674487810159No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24400.16107013944449286No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG23930.1579675588896194No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT23830.15730743536730593No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23720.1565812994927611No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23490.15506301539144005No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23370.15427086716466384No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23350.15413884246020115No Hit
GGCCAAAAGTGTACGGGAAACTGTTAGCCTGTTGACAATAGTAAGTTGCA23150.15281859541557416No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22830.150706200144171No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22650.1495179778040067No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22530.1487258295772305No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG22390.1478016566459916No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22270.1470095084192154No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21980.14509515020450628No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT21710.14331281669425983No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT20730.13684360617558758No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20160.13308090209840065No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGGAAA19690.1299783215435272No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18820.12423524689939981No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18310.120868616935601No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17680.11670983874502597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17120.1130131470200704No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC17000.1122209987932942No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16920.1116928999754434No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA16820.11103277645312991No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA16770.11070271469197315No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16460.10865633177280132No Hit
GATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC16420.10839228236387594No Hit
CTCCTGATCTATGCTGCATCCAATTTGCGAAGTGGGGCCCCATCAAGGTT16230.10713804767148029No Hit
GCTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTG16140.10654393650139814No Hit
GATCAGGAGCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCTAACC15980.10548773886569654No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15810.1043655288777636No Hit
GTCTCCTACAGATGCAGACACAGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG15650.10330933124206201No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC15590.10291325712867391No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT15330.10119693597065882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG306.954215E-445.1043242
TACGTAT1701.8189894E-1244.6323670
CGTATGC2450.038.0130141
AATGGTA15450.037.05575670
ATCTCGT2250.036.51503437
GTATCAA37850.036.110871
TCGTATG2600.035.7609640
TATCAAC40300.033.408542
ATGCCGT2700.033.3967644
CGAGACT3050.033.2857723
ACGAGAC3300.032.81072622
GAGACTA3250.032.28399324
GACTAGG3400.031.86991126
TCAACGC42250.031.7865374
TGTTAGA15700.031.63289370
ATCAACG42350.031.6316033
CACGAGA3350.031.30891821
CTTATAC3350.031.306851
CCCACGA3350.031.3037519
CAACGCA43100.031.159665
Produced by FastQC (version 0.11.5)