FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188981
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT42420.3567760965061679No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31410.26417579423052173No Hit
GTACAGGGGACTGGCCTGGCTTTTGCTGATACCAGCAAGCATATTTACTC31140.261904942131119No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29640.24928909713443698No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24530.20631111851240683No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24530.20631111851240683No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23030.1936952735157248No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22340.18789198481725106No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21470.1805747947191755No Hit
GCCTTGGGCTGACCTAGGACGGTCAGACTGGTCCCTCCGCCGAATACCAC19730.16594041452302433No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19720.16585630888971312No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA18680.15710932302534691No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18310.153997414592832No Hit
CCTATGACCTGACTCAGCCACCCTCACTGTCCGTGTCCCCAGGACAGACA18050.15181066812674046No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17050.1434001047956191No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16760.1409610414295939No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15990.13448490766463048No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15440.12985909783251373No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15200.1278405626330446No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGACTGGTCCCTC14850.12489686546715212No Hit
GTGGTATTCGGCGGAGGGACCAGTCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG14770.12422402040066242No Hit
GACCTAGGACGGTCAGACTGGTCCCTCCGCCGAATACCACAGTGCTGCTGTCCCACGTCTGACAGTAATAGTCAG14640.12313064716761664No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14610.122878330267683No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14350.12069158380159145No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13470.11329028807020465No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13460.11320618243689344No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13370.11244923173709252No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13260.11152406977066917No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13070.10992606273775611No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12720.10698236557186364No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12440.10462740783914967No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12370.10403866840597116No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12270.10319761207285903No Hit
GACTATTACTGTCAGACGTGGGACAGCAGCACTGTGGTATTCGGCGGAGGGACCAGTCTGACCGTCCTAGGTCAG12160.10227245010643567No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12100.10176781630656839No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11910.10016980927365533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAT12550.047.61998770
ACTCTGG19000.038.2756670
GTATCAA35250.037.100971
GGATGGG10000.033.84174770
TATCAAC39300.032.816132
GTATAAA1253.6379788E-1232.5258261
ATCAACG40000.032.2404943
TCAACGC39950.032.1961174
AACGCAG40750.031.81196
CAACGCA40600.031.5959645
GCGCTCA450.00417731331.30824952
AACTGTA2301.0768417E-931.30596470
GCGTAGA1153.2902212E-431.30596470
ACGCAGA42850.030.1738667
CGCAGAG42900.030.0598038
CAGAGTA43950.029.41866310
AGAGTAC44050.029.2738111
TTATCTC10600.029.23994357
TATCTCC10700.028.68961158
GCAGAGT45200.028.530219
Produced by FastQC (version 0.11.5)