FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170977
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT60330.5152108026032962No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50440.43075141527117955No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTTGGTCC49760.42494429864975997No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43820.3742174269861833No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG43340.37011828584165185No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC42500.36294478883872183No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG40930.34953718134515027No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC39230.3350193897916014No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37420.31956221172576404No Hit
GCCTTGGGCTGACCTCGGACGGTGACCTTGGTCCCTGTTCCGAAGACACT32750.2796809843404268No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT32510.27763141376816114No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29650.25320736444866127No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27870.23800638270435712No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27470.23459043175058092No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCGCCTCATATGCAGGCAGCAGCAGTGTCTTCGGAA27400.2339926403336701No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC27070.23117448079680472No Hit
GATTATTATTGCGCCTCATATGCAGGCAGCAGCAGTGTCTTCGGAACAGGGACCAAGGTCACCGTCCGAGGTCAG26470.2260505543661404No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25920.22135362180469814No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25910.22126822303085372No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTTGGTCCCTGTTCCGAAGACACTGCTGCTGC24780.21161816158643595No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC24240.20700662779883808No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23990.20487165845272792No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA23850.20367607561890627No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22630.19325742520988884No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21640.18480294659929272No Hit
ATAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG21350.18232638215780497No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCAATAAG21310.18198478706242735No Hit
GGATGAGGCTGATTATTATTGCGCCTCATATGCAGGCAGCAGCAGTGTCTTCGGAACAGGGACCAAGGTCACCGT19930.17019975627189945No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG19880.1697727624026774No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19660.16789398937810052No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA19100.16311165804281383No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18780.16037889727979288No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18660.15935411199365998No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18350.15670675000448345No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17790.15192441866919676No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAA17790.15192441866919676No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17730.15141202602613033No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17700.1511558297045971No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17540.1497894493230866No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATT17140.1463734983693104No Hit
CATATGCAGGCAGCAGCAGTGTCTTCGGAACAGGGACCAAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCA16850.14389693392782266No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16830.14372613638013385No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC16060.13715043079411465No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15980.13646724060335944No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC15750.1345030688049381No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15720.1342468724834049No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT15340.1310017190773175No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG15140.1292937436004294No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15090.12886674973120735No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15080.12878135095736296No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15030.1283543570881409No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14860.12690257793278606No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14740.12587779264665316No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14740.12587779264665316No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA14600.1246822098128315No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC14480.12365742452669864No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14330.12237644291903256No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG14230.12152245518058853No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14030.1198144797037004No Hit
CTTCGGAACAGGGACCAAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13790.1177649091314347No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13680.11682552261914622No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCA13480.11511754714225812No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA13370.11417816062996967No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13370.11417816062996967No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13060.11153079864079311No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13050.11144539986694871No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGC13050.11144539986694871No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG12970.11076220967619348No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12950.11059141212850467No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12820.10948122806852739No Hit
CAGCAGCAGTGTCTTCGGAACAGGGACCAAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCACTGTCACT12650.1080294489131725No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG12640.10794405013932809No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA12600.10760245504395048No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12540.10709006240088405No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG12390.10580908079321798No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT12250.1046134979593963No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12210.10427190286401866No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11980.10230773106559736No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11940.10196613597021974No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11890.10153914210099771No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11780.10059975558870926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.051.8044621
GTGGTAT2250.047.0529671
CCCTAAG1450.040.0788828
TAACGGC603.0254705E-740.0519436
GTATCAA29800.039.996041
ACGAGAC1400.039.02344522
TGGTATC2650.038.6569482
TTAACGG655.69853E-736.94405735
TCTCCGA1600.036.27651211
ATCTCCG1650.035.17722310
TATCAAC34300.034.8438452
TCGTATG709.830728E-734.45746640
ATATCGC400.002730649434.1469736
TATATCG501.9773986E-434.146975
ATCAACG35250.033.614183
TCAACGC35650.033.141244
CAACGCA36250.032.5926975
AACGCAG36800.032.1055766
AACGGCC652.3871E-531.71668237
CTTATAC1850.031.3823181
Produced by FastQC (version 0.11.5)