FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170977
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53490.45679804129372314No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC50960.4351921515110886No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTTGGTCC48920.41777080164682995No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC45650.38984540259970946No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG43400.3706306784847183No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG43110.36815411404323056No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42800.36550675205405403No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC41280.3525261384297044No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35630.30427583120761553No Hit
GCCTTGGGCTGACCTCGGACGGTGACCTTGGTCCCTGTTCCGAAGACACT33600.2869398801172013No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT32730.27951018679273804No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29990.25611092275937103No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC29190.2492790208518186No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28720.24526527848113158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27900.23826257902589038No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27470.23459043175058092No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC26960.23023509428451625No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG26360.22511116785385196No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTTGGTCCCTGTTCCGAAGACACTGCTGCTGC25450.21733987943401112No Hit
GATTATTATTGCGCCTCATATGCAGGCAGCAGCAGTGTCTTCGGAACAGGGACCAAGGTCACCGTCCGAGGTCAG25190.21511951131405654No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCGCCTCATATGCAGGCAGCAGCAGTGTCTTCGGAA24870.2123867505510356No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24350.2079460143111265No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23470.20043092221281888No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA23430.20008932711744123No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCAATAAG22600.19300122888835564No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21840.18651092207618084No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA19640.1677231918304117No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19580.16721079918734527No Hit
GGATGAGGCTGATTATTATTGCGCCTCATATGCAGGCAGCAGCAGTGTCTTCGGAACAGGGACCAAGGTCACCGT19250.1643926396504799No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19130.16336785436434706No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19080.16294086049512502No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18690.1596103083151932No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAA18580.15867092180290474No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18560.15850012425521595No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18300.1562797561352614No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17110.14611730204777718No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16990.14509251676164434No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16980.14500711798779992No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16910.1444093265708891No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC16570.14150576826017933No Hit
ATAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG16530.1411641731648017No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16370.1397977927832912No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16150.1379190197587143No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15700.13407607493571608No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15620.13339288474496083No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15560.1328804921018944No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15520.13253889700651678No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT15160.1294645411481182No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15020.12826895831429652No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATT14970.1278419644450745No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14670.12528000122974234No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14450.12340122820516541No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14350.12254724046672139No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14310.12220564537134375No Hit
CATATGCAGGCAGCAGCAGTGTCTTCGGAACAGGGACCAAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCA14250.12169325272827732No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT14200.1212662588590553No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13950.11913128951294519No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA13920.11887509319141196No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA13860.11836270054834552No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13820.1180211054529679No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG13760.11750871280990147No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG13640.11648392752376861No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG13310.11366576798690325No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13170.11247018515308158No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG13130.11212859005770394No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13080.11170159618848192No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA12900.11016441825928264No Hit
CTGCTGCTGCCTGCATATGAGGCGCAATAATAATCAGCCTCATCCTCAGC12830.10956662684237181No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG12780.10913963297314977No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12630.10785865136548368No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12530.10700466362703964No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12490.10666306853166202No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12480.10657766975781763No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12180.10401570654248546No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12170.10393030776864105No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG12080.1031617188040414No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCA12040.1028201237086638No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGC12030.10273472493481939No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG12000.10247852861328616No Hit
CTTCGGAACAGGGACCAAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11950.10205153474406414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG200.00772547651.117852
GGTATCA12250.047.0466651
GTATCAA32650.039.2719841
GTGGTAT3400.038.1138571
AACGCTA400.002257499635.49768461
AATGGTA3650.034.67861670
TGGTATC3650.034.5453952
TATCAAC36050.034.503962
CTATAAG501.9928112E-434.1018681
TAGTTCG601.4613019E-534.0756577
ATCAACG37900.032.9925163
TCAACGC38200.032.5578084
CAACGCA38950.032.0197455
ACGCTAA450.00397369731.62948662
AACGCAG39550.031.620146
GTTTCGC450.004292180831.13516854
TACGTTA450.004465473430.88434247
ATTACGT450.00451420630.81590345
TAGGCAA3251.8189894E-1229.7828170
CGCAGAG42100.029.623978
Produced by FastQC (version 0.11.5)