FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737199
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35120.4763978247393173No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33120.4492681080685134No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24650.33437375796765867No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24190.32813392313337375No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG23710.3216227911323808No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC23710.3216227911323808No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23440.31796027938182225No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21960.29788428904542735No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21410.29042361696095625No Hit
GATTATTACTGCAGCTCATATACAACTACCAGCCCCTTTGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGAGT20080.27238235537487165No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19890.2698050322911453No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT18600.2523063650384767No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT18330.2486438532879182No Hit
GCCTTGGGCTGACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCTGGTAGTTGTATATGAGCTG17070.23155213178531173No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16860.22870351153487728No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTCTGTCTCCTGGTATCAACAACACCCAGGCAAAGC16720.226804431367921No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGCTCAGGGAGG16700.226533134201213No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTCTGTCTCCTGGTATCAACAACA16420.22273497386730043No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTCAGGACGGTGACCTTGGTC16300.2211071908670522No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16220.22002200220022No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16000.21703773336643162No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15680.21269697869910295No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15380.20862752119848235No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15020.20374417219773763No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14480.19641914869662058No Hit
ACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCTGGTAG14380.19506266286308038No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14210.19275663694606204No Hit
GAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT13740.1863811535284231No Hit
GTTATAACTCTGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAAA13640.18502466769488293No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13510.18326123611128067No Hit
GTCCTGAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13470.1827186417778646No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13030.17675010411028771No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12780.17335888952643722No Hit
GCCCAGAGATGCTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAGAGAAG12320.16711905469215232No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11710.1588444911075571No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11140.151112521856378No Hit
GGTTATAACTCTGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT11090.1504342789396079No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10920.14812825302258956No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10480.1421597153550127No Hit
GCCTGGAGCCCAGAGATGCTCAGGGAGGCCGTGTTGTCAGACTTGGAGCC10340.14026063518805643No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10220.1386328521878082No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGATACCAGGAGA10210.13849720360445417No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10020.1359198805207278No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10020.1359198805207278No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA9830.1333425574370014No Hit
GAGCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAACTACCAGCCCCTTTGT9820.1332069088536474No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG9460.12832355985290267No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9330.12656012826930044No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9200.12479669668569818No Hit
CCCTGAGCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC9020.1223550221853258No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAATCGGCCCTCAGGGGTTTCTGATCG8940.12126983351849366No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8700.11801426751799718No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT8670.11760732176793512No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT8580.11638648451774894No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8570.11625083593439492No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC8510.1154369444342708No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8490.11516564726756276No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8470.11489435010085473No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8450.11462305293414668No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8450.11462305293414668No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8430.11435175576743864No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8390.11380916143402256No Hit
GACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCTGGTAGTTGTATATGAGCTGCAGTAATAAT8380.11367351285066854No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT8340.11313091851725247No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8190.11109618976694216No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8020.10879016384992383No Hit
GAGATGCTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAGAGAAGCGATC7920.10743367801638362No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAACTA7780.10553459784942736No Hit
GGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC7640.10363551768247108No Hit
CAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATA7600.10309292334905501No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC7570.10268597759899294No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC7510.10187208609886882No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG7500.10173643751551481No Hit
GTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCC7480.10146514034880677No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA7460.10119384318209873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG306.7057234E-445.4355932
TCGATAA404.2346262E-544.3562859
CGTATGC602.93041E-740.2117341
CGATAAC458.303499E-539.5703760
GTCGATA458.6421365E-539.30285658
GTGGTAT2000.039.190861
ACCGTAC754.433241E-836.39044627
ACGAGAC853.3469405E-936.08120722
TCGTATG709.913401E-734.41760340
TCTCGTA701.0079311E-634.3446538
CTAGATC807.6181095E-834.21830733
CGTACTA905.7534635E-934.1345929
GACCGTA807.849303E-834.10214626
TGCATCG400.002760462734.0697616
AGACCGT959.858923E-932.3007225
ATCTCGT851.2660348E-732.2956737
ACTAGAT851.3037425E-732.1879232
GTCGCCC703.0119688E-431.78128869
TACTAGA1001.5865226E-830.7608531
GAGACCG1001.6290869E-830.67735324
Produced by FastQC (version 0.11.5)