FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316973
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48980.3719134712708613No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34510.2620402999909641No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG34430.2614328463833351No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACATAATTCAACTTCAGGGAGA32080.24358889665923295No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30980.23523640955433406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27860.21154571885680268No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAT26640.2022820513404603No Hit
CCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAATGTACACTGGTACCAGCAGAGGCCAGGCCAGGCCC26210.19901698819945435No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25940.19696683227370645No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25380.19271465702030338No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25060.19028484258978734No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC24700.1875513013554568No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24400.18527335032684802No Hit
GGGTGGCCGTGTCCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGACCCCTGAGGGCCGGTCGCTACCATTATAGA23330.17714865832481No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22920.17403545858571132No Hit
CCCCTGTGCTGGTCGTCTATAATGGTAGCGACCGGCCCTCAGGGGTCCCT22720.1725168245666388No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21570.1637846789569718No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20920.15884911839498608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20660.1568748941701918No Hit
CGGCCACCCTGACCATTAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT20460.15535626015111928No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20010.1519393336082061No Hit
CCCCAGGACAGACGGCCACCATTACCTGTGGGGGAAACAACATTGGAAGT20000.15186340190725248No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACATTTTTACTT19620.14897799727101466No Hit
CACCTGCACTGTCTCCGGTGTCTCCCTCAGTGGTTACTATTGGAGCTGGA19170.14556107072810148No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18690.14191634908232745No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC18660.14168855397946656No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18540.14077737356802303No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCATATAAGGACCACCGTCACTAACCCA18380.139562466352765No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATAATGGTAGCGACCGGCCC18160.13789196893178524No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18090.13736044702510986No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC17660.13409538388410394No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT17510.13295640836979952No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17000.1290838916211646No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATAATGGTAGCGACCGGCCCTCAGG16840.12786898440590658No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16580.1258947601811123No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16440.12483171636776151No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16370.12430019446108614No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16150.12262969704010637No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTAC16120.12240190193724548No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15970.12126292642294109No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15890.12065547281531208No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAACTCTGGGGACACGG15770.11974429240386855No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15750.11959242900196132No Hit
GTGTATTACTGTGCGAGGTATTATTGTATTAGTACCAGCTGCTATGTAAC15470.11746634137525978No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15220.11556804885141912No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14920.11329009782281034No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14840.11268264421518133No Hit
GTCTCCGGTGTCTCCCTCAGTGGTTACTATTGGAGCTGGATCCGGCAGTC14750.11199925890659869No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14500.11010096638275804No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14390.10926571767226816No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACATAATTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTA14370.1091138542703609No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14050.10668403983984487No Hit
CCATATAAGGACCACCGTCACTAACCCACACCTGACAGTAATAGTCGGCC13870.10531726922267959No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACATTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGGTGGC13630.10349490839979257No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG13590.10319118159597807No Hit
CTATTACTGTCAGGTGTGGGTTAGTGACGGTGGTCCTTATATGGTATTCGGCGGAGGGACCAAGCTGACCGTC13480.10235593288548815No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA13480.10235593288548815No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA13290.10091323056736926No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13220.10038170866069387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)