FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728225
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT87650.5071677588276989No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79810.4618032952885186No Hit
GTGCAGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT57910.33508368412677747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57260.3313225997772281No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56750.3283715951337355No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56130.3247840992926268No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC45520.26339163014075134No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44770.2590519174297328No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA43910.25407571352109826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42300.24475979690144511No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41100.2378162565638155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40470.2341708978865599No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37620.2176799895846895No Hit
GGACTGCACCTTCCAAAATAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGG37320.21594410450028206No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36880.21339813970981786No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA36550.21148866611696973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35990.20824834729274255No Hit
GTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT34710.2008419042659376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34430.19922174485382402No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34360.1988167050007956No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG32350.18718627493526596No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31990.18510321283397704No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31750.18371450476645113No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30870.17862257518552271No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30740.17787035831561285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30560.1768288272649684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30130.1743407253106511No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC29490.17063750379724862No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC28920.16733932213687455No Hit
CCATGATAGTGTTGGCAGAAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC28280.16363610062347206No Hit
ATTTTGGAAGGTGCAGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC28130.16276815808126835No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27700.16028005612695106No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAA27700.16028005612695106No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27490.15906493656786588No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27400.15854417104254365No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACTGCA26360.15252643608326463No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT26020.15055909965426956No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25890.1498068827843597No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25180.1456986214179288No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24730.14309479379131768No Hit
CTATCATGGTTATTCTCTTAGGTGGACGTTCGGCCAAGGGACCAAGGTGG24580.14222685124911397No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23630.13672988181515716No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23400.13539903658377817No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23310.13487827105845593No Hit
GTCTCCTTCCTCTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGA22960.13285307179331393No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22850.1322165805956979No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22410.1296706158052337No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22250.12874481042688307No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCTCT21810.12619884563641887No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGA21370.12365288084595467No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21080.12197485859769418No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20710.11983393366025835No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20640.11942889380722997No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20590.11913957962649539No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20410.11809804857585095No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20320.11757728305052872No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20070.11613071214685589No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19730.11416337571786082No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19460.11260107914189414No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTCACTA19080.11040229136831142No Hit
CTCTTAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACT19050.11022870285987067No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18770.1086085434477571No Hit
GTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGA18730.10837709210316944No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18310.10594685298499905No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18190.10525249895123609No Hit
TCTATAAGGCGTCTATTTTGGAAGGTGCAGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT18170.10513677327894227No Hit
CCTTGGTCCCTTGGCCGAACGTCCACCTAAGAGAATAACCATGATAGTGTTGGCAGAAATAAGTTGCAAAGTCAT18130.1049053219343546No Hit
CACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTATTTCTGCCAAC18040.1043845564090324No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17950.10386379088371017No Hit
TGGTTATTCTCTTAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT17880.10345875103068176No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17630.10201218012700893No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17510.10131782609324597No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17390.10062347205948298No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTCACTAATACTCTGA17310.10016056937030768No Hit
TTCCAAAATAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT17300.10010270653416078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1103.092282E-1158.0678670
GTACGTA1201.5097612E-1037.6251469
GTGGTAT5500.035.0425031
GGTACGT1252.5465852E-1134.78143368
GTATCAA40250.033.6869281
AATGGTA9000.030.75445770
TGGTACG1251.6025297E-930.17420467
TATCAAC44800.030.0252652
ATCAACG46650.028.8312153
TCAACGC47300.028.3627474
CAACGCA47700.028.1281625
AACGCAG47900.027.7990476
CGGTACC906.5078457E-627.04050845
GCTTCCG1900.026.69906617
TGTTAGA24600.026.54230970
CGCAGAG51650.026.0424568
ACGCAGA51650.025.9115897
AGAGTAC51300.025.89073611
GACAGGT2750.025.8207931
AATAGTC2150.025.45502736
Produced by FastQC (version 0.11.5)