FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013520
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT24310.23985713158102456No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22050.2175586076249112No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18480.18233483305706844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15700.15490567527034493No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15340.15135369800299944No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13570.13388980977188414No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13210.13033783250453865No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13190.13014050043413056No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC11480.11326860841423948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.11277527823821927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10520.10379666903465151No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA10410.10271134264740706No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10150.10014602573210198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG2650.045.8940627
GGTATCA20150.043.1597561
CTAGCGC2950.042.40141328
TAGCGCT3000.041.72363329
TCGTATG1700.040.56133740
CCTAACG751.0950316E-940.4457322
GTATCAA52600.039.2833981
GACTAGC3200.037.97971726
CGTATGC1800.036.45693641
CTAACGC1052.1827873E-1135.3080253
ATCAACG61200.033.8683243
CTTATAC4100.033.7081031
TCAACGC61650.033.6211134
GTGGTAT4250.033.3115351
TATCAAC62300.033.2178732
CAACGCA62200.033.2154465
CGAGACT3800.032.8323923
ACGAGAC3850.032.39959722
AGACTAG3750.032.3869525
GAGACTA3750.032.37575524
Produced by FastQC (version 0.11.5)