FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974105
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGTCG38680.3970824500438864No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38480.39502928329081566No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34470.35386328989174676No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31190.3201913551413862No Hit
CCCCAGGACAGTCGGCCATGATTACCTGTGGGGGAAACAATATTGGAAGT30340.31146539644083543No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC29320.30099424600017455No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28140.288880562157057No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26820.27532966158678995No Hit
GACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTACTACCATCCCACACCTGACAGTAATAGT25850.2653718028343967No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25720.2640372444449007No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22950.23560088491487058No Hit
CCCCTGTGCTGGTCGTCTATGATGGTCACGACCGGCCCTCAGGGATCCCT22920.23529290990190996No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22460.23057062636984718No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTACTACCATCCCACACCTGA21180.21743035915019426No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21110.2167117507866195No Hit
ACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTACTAC20680.21229744226751737No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT20580.211270858890982No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20450.20993630050148598No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTA20290.20829376709902936No Hit
GTAGTAGTGATCCCCTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA20020.20552199198238383No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19310.19823325000898262No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19180.1968986916194866No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17360.17821487416654264No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16400.16835967375180294No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16070.16497194860923617No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15950.1637400485573937No Hit
CTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCAC15540.15953105671359863No Hit
CACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCACGACCCTGCTGATGGTCAGGGTGG15440.15850447333706325No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15010.1540901648179611No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT14910.15306358144142573No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA14590.14977851463651248No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14470.14854661458467003No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14470.14854661458467003No Hit
TGGTAGTAGTGATCCCCTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA14440.1482386395717094No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG14280.1465961061692528No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC14250.1462881311562922No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14220.14598015614333157No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGTCGGCCATGATTAC13800.14166850596188296No Hit
CGGCCACCCTGACCATCAGCAGGGTCGTGGCCGGGGATGAGGCCGACTAT13530.1388967308452374No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA13200.13550900570267066No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13150.13499571401440297No Hit
CAGTAATAGTCGGCCTCATCCCCGGCCACGACCCTGCTGATGGTCAGGGT12950.1329425472613322No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12940.13283988892367865No Hit
CTATTACTGTCAGGTGTGGGATGGTAGTAGTGATCCCCTCTTCGGATCTG12880.13222393889775744No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12680.13017077214468667No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG12460.1279122887163088No Hit
GTAATAGTCGGCCTCATCCCCGGCCACGACCCTGCTGATGGTCAGGGTGG12370.12698836367742697No Hit
ACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAA12270.12596178030089158No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12130.12452456357374204No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC12120.12442190523608851No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12090.12411393022312789No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGGTCACGACCGGCCCTCAGG11700.12011025505463989No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11130.1142587298083882No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG10930.11220556305531744No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10880.11169227136704975No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGTGACCATCATAGA10880.11169227136704975No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA10880.11169227136704975No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10870.11158961302939621No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC10760.1104603713152073No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10750.11035771297755374No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10750.11035771297755374No Hit
CTTTTACTTCCAATATTGTTTCCCCCACAGGTAATCATGGCCGACTGTCCTGGGGCCACTGACACCGAGGGTGG10680.10963910461397898No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10520.10799657121152238No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10520.10799657121152238No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10500.10779125453621528No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10330.10604606279610515No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGTCGGCCATGATTACCTGTGGGGGAAACAATATTGGAAGTAAAAGTGTG10280.10553277110783746No Hit
AAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC10200.10471150440660915No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGTCGG10180.10450618773130207No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10030.102966312666499No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG9970.10235036264057776No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9800.10060517090046761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)