FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974105
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34780.3570456983590065No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34700.3562244316577782No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGTCG34280.35191278147632954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA31600.3244003469851813No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29910.3070510879217333No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28290.29042043722186006No Hit
CCCCAGGACAGTCGGCCATGATTACCTGTGGGGGAAACAATATTGGAAGT26140.2683488946263493No Hit
GACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTACTACCATCCCACACCTGACAGTAATAGT24470.2512049522382084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23790.2442241852777678No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23470.24093911847285457No Hit
CCCCTGTGCTGGTCGTCTATGATGGTCACGACCGGCCCTCAGGGATCCCT22550.23149455140872902No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21730.2230765677211389No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21020.2157878257477377No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21010.21568516741008412No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTACTACCATCCCACACCTGA20370.20911503380025767No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20290.20829376709902936No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19600.20121034180093522No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT19600.20121034180093522No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTA19470.1998757834114392No Hit
GTAGTAGTGATCCCCTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA18960.19464020819110875No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18700.19197109141211677No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18640.19135514138619555No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18410.18899399962016417No Hit
ACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGAGGGGATCACTACTAC18000.18478500777636908No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGTGACCATCATAGA16190.16620384866107865No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16150.16579321531046448No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT16060.16486929027158265No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT15880.16302144019381895No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC15850.16271346518085833No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGTCGGCCATGATTAC15280.15686193993460665No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15270.1567592815969531No Hit
CACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCACGACCCTGCTGATGGTCAGGGTGG15060.15460345650622878No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14780.1517290230519297No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG14520.14905990627293772No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA14230.14608281448098512No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14180.14556952279271743No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14030.14402964772791435No Hit
CTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCAC13890.1425924310007648No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC13840.1420791393124971No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC13610.13971799754646574No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13510.13869141416993036No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13330.13684356409216666No Hit
TGGTAGTAGTGATCCCCTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA12930.13273723058602513No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGGTCACGACCGGCCCTCAGG12890.13232659723541096No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG12840.13181330554714327No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12830.13171064720948975No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA12690.1302734304823402No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12300.1262697553138522No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12180.12503785526200975No Hit
CGGCCACCCTGACCATCAGCAGGGTCGTGGCCGGGGATGAGGCCGACTAT12110.12431924689843497No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA11830.12144481344413591No Hit
CTATTACTGTCAGGTGTGGGATGGTAGTAGTGATCCCCTCTTCGGATCTG11700.12011025505463989No Hit
ACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAA11560.11867303832749035No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11480.11785177162626206No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG11410.11713316326268729No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11400.11703050492503375No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11120.11415607147073467No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11100.1139507547954276No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11030.11323214643185282No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC10840.11128163801643559No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10630.10912581292571129No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10620.10902315458805777No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC10540.10820188788682944No Hit
CAGTAATAGTCGGCCTCATCCCCGGCCACGACCCTGCTGATGGTCAGGGT10450.10727796284794759No Hit
CTTTTACTTCCAATATTGTTTCCCCCACAGGTAATCATGGCCGACTGTCCTGGGGCCACTGACACCGAGGGTGG10400.10676467115967991No Hit
GTAATAGTCGGCCTCATCCCCGGCCACGACCCTGCTGATGGTCAGGGTGG10390.10666201282202638No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10170.10440352939364853No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10150.10419821271834145No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGTCGGCCATGATTACCTGTGGGGGAAACAATATTGGAAGTAAAAGTGTG10060.1032742876794596No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10040.10306897100415253No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG10000.10265833765353838No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9980.1024530209782313No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9960.10224770430292422No Hit
AAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC9940.10204238762761715No Hit
GATTACCTGTGGGGGAAACAATATTGGAAGTAAAAGTGTGCACTGGTACC9850.1011184625887353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAA200.0065928553.20820259
CACGAGA200.007570933651.3791334
TCGCTAC200.00778039851.02568411
GGTATCA11150.046.1011621
GTGGTAT3100.039.5321661
GTATCAA34100.038.533881
TCTCGAT2650.037.3002870
TGGTATC3200.036.1506162
TATCAAC36150.035.8594362
ATCAACG38900.033.317543
TCAACGC39050.033.100754
AACGCAG39750.032.6906666
CAACGCA39800.032.564135
GAATACG1850.031.2589769
ACGCAGA42600.030.5036167
CGCAGAG42950.030.175848
TACGGGC1501.8189894E-1229.47999215
ACGTACC1850.029.41719413
AGAGTAC45600.028.94439111
GCAGAGT45300.028.9107999
Produced by FastQC (version 0.11.5)