FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241658
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41090.330928484333045No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40140.323277424218263No Hit
GTATAATAATTGGCCTTCCGCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT37260.30008263144923963No Hit
GTATGGGACAGAGTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATT31430.25312928358694586No Hit
TTATTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAACTCTGTC28930.23299491486383528No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26920.21680688241045443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24440.19683358863712874No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22670.18257845558116648No Hit
GTCCCATACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT22610.18209523073181183No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC22510.18128985598288738No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21540.1734777209183205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20750.16711526040181757No Hit
GAGTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAATAATTGG20320.16365214898144256No Hit
CATCTATGGTGCCTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTATGGGACAGAGTTCA19740.15898097543768092No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19560.15753130088961695No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18710.15068561552375936No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18290.1473030415782768No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTATGGGACAGAGTTCACT17850.14375939268300933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17580.1415848808609134No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17330.13957144398860233No Hit
GTATAAAGAGACCGATCTCTCGCACAGTAATATACACCCGTGTCTTCAGA17140.13804123196564594No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16990.1368331698422593No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT16850.1357056451937651No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC16810.13538349529419533No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGCG16780.13514188286951803No Hit
GTTTATTACTGTCAACAGTATAATAATTGGCCTTCCGCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA16670.13425597064570116No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16540.1332089834720994No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16430.13232307124828255No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16190.1303901718508639No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15630.12588007325688716No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15440.12434986123393076No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15440.12434986123393076No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTACCAGTAACTTAGCCTGGTA15320.12338341153522146No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13760.11081956545200047No Hit
GTAATAAACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCATACCCACTGCCA13690.11025580312775338No Hit
GAGTGAACTCTGTCCCATACCCACTGCCACTGAACCTGGCTGGGATACCA13460.10840344120522721No Hit
GGGTATGGGACAGAGTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGA13350.10751752898141033No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13300.10711484160694812No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13270.10687322918227081No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13170.10606785443334638No Hit
GTCAGAGTCTTACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG13040.10502086725974462No Hit
GTATATTACTGTGCGAGAGATCGGTCTCTTTATACAGGGCAAGACTACTGGGGCCAGGGAACCCTGGTCACCGT12880.10373226766146557No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12870.10365173018657313No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG12840.10341011776189579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC401.3864337E-859.253463
CCTAACG553.0668161E-949.2516172
GGTATCA14100.041.0664981
TCGTATG1250.038.60317640
GTATCAA38150.036.6577841
CGTATGC1700.036.5530441
ATGCCGT1450.033.4969544
TCTCCGA3200.032.79706211
ATCTCGT959.311407E-932.49453437
CGAGACA3150.032.2571823
GAGCGAT2750.032.23686632
CCGATCT5900.032.1335912
CTTATAC3400.031.8699761
ACGAGAC3300.031.8134622
ATCAACG43850.031.8129043
TCAACGC43900.031.776674
GTCGTAT652.3651255E-531.75981539
TCTCGCA5900.031.5648618
GACCGAT5800.031.520210
ACCGATC5800.031.520211
Produced by FastQC (version 0.11.5)