FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241658
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41630.3352775079772369No Hit
GTATAATAATTGGCCTTCCGCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT35410.2851831985941378No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33650.271008603013068No Hit
GTATGGGACAGAGTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATT29020.23371975213786725No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28790.23186739021534108No Hit
TTATTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAACTCTGTC28440.22904857859410563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27940.2250217048494835No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC24470.19707520106180607No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22550.18161200588245716No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22150.17839050688675948No Hit
GTCCCATACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT21460.17283342111918096No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21100.16993407202305305No Hit
GAGTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAATAATTGG20330.16373268645633499No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19560.15753130088961695No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18760.15108830289822156No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT17740.14287348045919246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17620.14190703076048314No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17500.14094058106177387No Hit
CATCTATGGTGCCTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTATGGGACAGAGTTCA17380.13997413136306455No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17350.1397325189383872No Hit
GTATAAAGAGACCGATCTCTCGCACAGTAATATACACCCGTGTCTTCAGA17310.13941036903881743No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTATGGGACAGAGTTCACT17100.13771908206607616No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16940.1364304824677971No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16870.13586672014355No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16800.1353029578193029No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC16560.1333700584218843No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16370.13183984639892787No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGCG16090.12958479710193951No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15980.12869888487812264No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15910.12813512255387555No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15820.12741028527984358No Hit
GTTTATTACTGTCAACAGTATAATAATTGGCCTTCCGCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA15820.12741028527984358No Hit
GAGTGAACTCTGTCCCATACCCACTGCCACTGAACCTGGCTGGGATACCA15780.1270881353802738No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15620.1257995357819947No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15030.12104782476334063No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTACCAGTAACTTAGCCTGGTA14500.11677933859404119No Hit
GTAATAAACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCATACCCACTGCCA14430.1162155762697941No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14070.11331622717366618No Hit
GGGTATGGGACAGAGTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGA13430.10816182878054988No Hit
GTCAGAGTCTTACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG13330.10735645403162546No Hit
GTATATTACTGTGCGAGAGATCGGTCTCTTTATACAGGGCAAGACTACTGGGGCCAGGGAACCCTGGTCACCGT12850.10349065523678823No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12820.10324904281211089No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12790.10300743038743358No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12570.10123560593979986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG457.2759576E-1267.598312
CGCATAA553.7815486E-451.03113670
CTAACGC652.6193447E-1046.7912943
GGTATCA15650.042.34871
GTATCAA40250.037.132551
TACGTAT1201.1957953E-535.08390470
GGCCTAT850.00321751733.02014570
CCGCATA550.00339182932.66834369
ATCAACG45450.032.6412353
TATCAAC45200.032.6027262
TCAACGC46050.032.2945254
AACGCAG46500.031.9832886
CAACGCA46800.031.7769855
ACGCAGA48750.030.5071357
CGCAGAG48650.030.5003668
GACGTTA450.005070768830.09218625
CGGGTAT902.3810207E-730.05459417
AGAGTAC49650.029.74750711
CTAATCG500.007467591727.79975145
GCAGAGT53500.027.7353789
Produced by FastQC (version 0.11.5)