FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759145
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35980.4739542511641386No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13080.17229909964499535No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12220.16097056557047731No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11900.1567552970776334No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11590.15267175572519084No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11360.14964203149595928No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10910.14371431017789751No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10780.14200185735267967No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8790.11578815641280651No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8620.1135487950259832No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT8470.11157288791996259No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8230.10841143655032964No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8190.10788452798872415No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8160.10748934656752004No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8120.10696243800591455No Hit
CCTATATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC8110.10683071086551317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG200.00897228449.2172242
CTAACGC1150.045.6537633
CCTAACG1150.045.650762
GTATCAA50250.035.015061
GGTATCA21550.032.2934761
ATGGCAA4150.032.26101370
TATCAAC56600.031.1303312
CTGCGGT553.427298E-431.09034238
AACGCAG57700.030.4211226
ATCAACG58700.029.739113
CAACGCA59200.029.6503145
TCAACGC59000.029.639614
CGCAGAG59050.029.5589498
TACCCTT1800.029.1619214
AGAGTAC60100.028.7695711
ACGCAGA61550.028.6248667
GCAGAGT60600.028.3697789
CAGAGTA62500.027.82230410
TGTACCC1900.027.6270812
ACGCCAT1950.026.9204676
Produced by FastQC (version 0.11.5)