FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361479
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17050.12523145784841339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG1800.048.714842
CTAACGC1750.048.1706663
TAACGCC1800.043.0891464
GGTATCA20250.036.812181
GTATCAA48700.036.2240521
TATCAAC55700.031.6672
TCAACGC55650.031.5707684
ATCAACG56300.031.2638763
CAACGCA57650.030.5924995
AACGCAG58050.030.3247226
CGCAGAG61250.028.3550018
ACGCAGA62450.028.080157
AGAGTAC62050.028.04377211
CAGAGTA62550.027.81960110
CCGGTGA3850.027.67904353
TCCCGCA6150.027.25700470
GCAGAGT64750.026.7181369
GCCACGT4900.026.24383469
AACGCCA3100.026.107315
AGTACGG38150.025.5390213
Produced by FastQC (version 0.11.5)