FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812457
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCGAGGGCGGTCAGCTGGGTGCCTCCTCCGAACACAGGGGGAGTGATACTACCTGCATATGAG72980.40265782857193305No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC72050.39752667235691663No Hit
GTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAATTGATTCTTGGGCGTGTCTACGGA70900.3911816942415738No Hit
CTCTATTACTGTGCGAGACAAGGCGACACCTACTGTAGTAGTACCAACTG51930.28651714219978736No Hit
CAGTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGA42570.23487453771317057No Hit
CTATTACTGTGCGAGACAAGGCGACACCTACTGTAGTAGTACCAACTGCCACCCCTCCCACTTTGACTACTGGGG39760.2193707216226371No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT36560.20171513034516128No Hit
CACTTGTACTGTCTCTGGTGGCTCCATCAGCGATCGGAGCCACTACTGGG35490.1958115420117553No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCACCATCTCCTG35140.19388046171578138No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGGCGGTCAGCTGGGTGCCTC34680.19134247046964425No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31950.17628004416104767No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG30860.17026610838215747No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT30300.1671763799085992No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29940.16519012588988316No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGGAATTATGACCTTGTCTCCTGGTACCAACAACA29870.16480390983068838No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGGAATTATGACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC29580.16320387187116714No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29330.16182452880261433No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT28950.1597279273384141No Hit
GTCTCTGGTGGCTCCATCAGCGATCGGAGCCACTACTGGGCCTGGGTCCG28350.15641750397388737No Hit
ACTTACTACAACCCGTCCCTCAAGAGTCGACTCACCATGTCCGTAGACACGCCCAAGAATCAATTCTCCCTGAGG27760.1531622543321028No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCAC27220.15018287330402874No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27110.1495759623538655No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26930.14858283534450747No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG26820.14797592439434426No Hit
CTACTGGGCCTGGGTCCGCCAGCCCCCAGGGAAGACACTGGAGTGGATTGGGACCATCTACCATAGCGGGACTA26420.14576897548465978No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26040.14367237402045951No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25610.1412999039425487No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCACCATCT25140.13870673897366945No Hit
ATCTACCATAGCGGGACTACTTACTACAACCCGTCCCTCAAGAGTCGACT23740.1309824177897738No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC23390.12905133749379985No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23180.1278926893162155No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA23050.12717543092056804No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22590.1246374396744309No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22330.12320292288313599No Hit
GTCCTATCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC21960.12116149514167784No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21860.12060975791425672No Hit
CCCCTGAGGGCCGCTCACTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA21570.11900971995473547No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21130.11658207615408256No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21020.11597516520391932No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21010.1159199914811772No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20880.11520273308552975No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20850.11503721191730343No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20720.11431995352165596No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC20370.11238887322568204No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20300.11200265716648726No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCAC20250.11172678855277669No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG20210.11150609366180825No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20140.11111987760261346No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC20010.11040261920696602No Hit
GAGCCACTACTGGGCCTGGGTCCGCCAGCCCCCAGGGAAGACACTGGAGT19960.11012675059325545No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19750.1089681024156711No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG19740.10891292869292898No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC19680.10858188635647632No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19560.10791980168357096No Hit
GTAATAATAAGCCTCGTCCTCAGCCTGGAGGCCAGAGATTGTCAGGGAGG19200.10593354766485494No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19100.10538181043743383No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT18870.10411281481436527No Hit
GCCTGGAGGCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18650.10289899291403878No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGTTGACCAGGGTTCCCTGG18440.10174034473645444No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTGGGAGGGGTGGCAGTTGGTACTACTACAGTAGGTGTCGCCTTGTCTCG18390.10146447612274388No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGCCTCCAGG18380.10140930240000176No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18360.10129895495451753No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18340.1011886075090333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG501.9065374E-458.62376870
CGAAAAG450.00923975348.85313870
GGTATCA15200.048.1067771
GTGGTAT3250.042.9053961
CGTATGC3100.041.1459141
TCGCTAA3800.040.40028831
CGCTAAT3500.040.0007632
AATCTCG2650.038.77620336
CTCGCTA4050.038.71452330
ATCTCGT2600.038.2459537
GCTAATC3050.038.0855433
CACGAGA4150.037.69720521
ACTCGCT4200.037.30401229
CACTCGC4250.036.84884628
GTATCAA36650.036.7479061
TGGTATC3800.036.693382
TCGTATG3200.036.57296440
CTACGGA23650.036.0126869
ACACTCG4450.035.9488427
GTATGCC3850.035.88597542
Produced by FastQC (version 0.11.5)