FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812457
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAATTGATTCTTGGGCGTGTCTACGGA75650.4173892125440769No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC69790.3850574110171993No Hit
GCCTTGGGCTGACCGAGGGCGGTCAGCTGGGTGCCTCCTCCGAACACAGGGGGAGTGATACTACCTGCATATGAG67900.37462957741894015No Hit
CTCTATTACTGTGCGAGACAAGGCGACACCTACTGTAGTAGTACCAACTG48560.2679235976356957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44230.24403337568836117No Hit
CAGTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGA40730.22472257272862198No Hit
CTATTACTGTGCGAGACAAGGCGACACCTACTGTAGTAGTACCAACTGCCACCCCTCCCACTTTGACTACTGGGG38330.21148087927051512No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCACCATCTCCTG36360.20061165589031907No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT34570.190735559519481No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGGCGGTCAGCTGGGTGCCTC31980.17644556532927402No Hit
CACTTGTACTGTCTCTGGTGGCTCCATCAGCGATCGGAGCCACTACTGGG30870.17032128210489958No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG30180.16651429523569386No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGGAATTATGACCTTGTCTCCTGGTACCAACAACA29830.16458321493971995No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT29410.16226591858455125No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29350.16193487624809857No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGGAATTATGACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC29130.16072105434777212No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29030.16016931712035098No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCAC28750.15862445288357185No Hit
GTCTCTGGTGGCTCCATCAGCGATCGGAGCCACTACTGGGCCTGGGTCCG28540.1574658047059875No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT27690.152776038272908No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27600.15227947476822898No Hit
ACTTACTACAACCCGTCCCTCAAGAGTCGACTCACCATGTCCGTAGACACGCCCAAGAATCAATTCTCCCTGAGG26090.14394824263417008No Hit
GTCCTATCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC25820.14245855212013306No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG25660.14157577255625925No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25630.14141025138803293No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC25430.14030677693319069No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24630.13589287911382172No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCACCATCT24570.13556183677736908No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24560.13550666305462694No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGGCGG24540.13539631560914273No Hit
CTACTGGGCCTGGGTCCGCCAGCCCCCAGGGAAGACACTGGAGTGGATTGGGACCATCTACCATAGCGGGACTA24330.13423766743155838No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23360.12888581632557353No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23290.12849960026637874No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23240.12822373165266818No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC22690.125189176901852No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC22570.12452709222894667No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA22540.12436157106072032No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22140.12215462215103587No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTGGGAGGGGTGGCAGTTGGTACTACTACAGTAGGTGTCGCCTTGTCTCG21960.12116149514167784No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC21450.11834763528183014No Hit
CCCCTGAGGGCCGCTCACTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA21420.1181821141136038No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21250.1172441608269879No Hit
ATCTACCATAGCGGGACTACTTACTACAACCCGTCCCTCAAGAGTCGACT21140.11663724987682467No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20680.11409925863068753No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC20500.1131061316213295No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG20340.1122233520574557No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT20120.11100953015712925No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19510.10764393306986042No Hit
GTATCACTCCCCCTGTGTTCGGAGGAGGCACCCAGCTGACCGCCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA19290.10643011116953395No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19270.10631976372404972No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGTTGACCAGGGTTCCCTGG19230.10609906883308129No Hit
GAGTGATACTACCTGCATATGAGCAGCAGTAATAATAAGCCTCGTCCTCA19160.1057128527738865No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19010.10488524693275482No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18890.10422316225984947No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCCCTGGACAGTCGATCAC18830.10389211992339681No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18550.10234725568661768No Hit
GCCTGGAGGCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18530.10223690824113345No Hit
GTGTTCGGAGGAGGCACCCAGCTGACCGCCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC18470.10190586590468077No Hit
GTAATAATAAGCCTCGTCCTCAGCCTGGAGGCCAGAGATTGTCAGGGAGG18240.10063687028161221No Hit
GAGCCACTACTGGGCCTGGGTCCGCCAGCCCCCAGGGAAGACACTGGAGT18240.10063687028161221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)