FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274055
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37650.29551314503690973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29790.23382036097342734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29020.2277766658425264No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28890.22675630172951716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26520.208154279053887No Hit
GTTACAATACCCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA26360.20689844629941406No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23460.18413647762459234No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCA22320.17518866924897278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21650.1699298695896174No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20400.16011867619529768No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19970.1567436256676517No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19240.151013888725369No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTAGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT19020.14928711868796873No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18600.1459905577074773No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18310.14371436083999514No Hit
GAGTTACAATACCCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18050.14167363261397664No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17930.14073175804812194No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17920.14065326850096738No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17810.13978988348226723No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17750.1393189461993399No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17080.13406014653998455No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16760.1315484810310387No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16730.13131301238957502No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16400.1287228573334746No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15490.12158030854240987No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15060.11820525801476388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14880.11679244616598185No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC14750.11577208205297258No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAACAAATATTTAAATTGGTATCAGCAGAAA14290.11216156288386295No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13910.10917896009198975No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13900.10910047054483518No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13900.10910047054483518No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCTACTTACTACTGTCAACAGAGTTACAATACC13880.10894349145052608No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13560.10643182594158022No Hit
GTAGTAAGTAGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCT13320.10454807680987084No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13110.10289979631962513No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT13070.1025858381310069No Hit
GTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCTACTTA13060.10250734858385235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG405.6010827E-542.3064882
GTATCAA49000.037.2443351
GTGGTAT4050.035.1125031
TATCAAC52900.034.101112
CAACGCA55000.032.5439765
AACGCAG56600.032.1009946
TCAACGC56600.031.6240064
ATCAACG56750.031.5391793
ACGCAGA59550.030.5107657
CGCAGAG59600.030.4851688
CCGTTAA450.005077064530.08461616
AGAGTAC61250.029.93896111
GCAGAGT62650.029.1630739
GGTATCA27800.027.403451
CAGAGTA67650.027.20769110
CTAACGC659.700074E-426.0265883
ATAAACG1307.763447E-926.0265883
TACGTAT1609.985192E-525.7079370
GAGTTTA7000.025.152021
GAGTACG46900.024.45419312
Produced by FastQC (version 0.11.5)