FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098267
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCA45970.4185685265969022No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45650.4156548453153924No Hit
CCCAAGAACCACTCAGCCTGCTGTCAAAGGACTGGCAGTAATAATCAGCC43580.3968069695256254No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36820.33525545245372934No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36390.3313401932317005No Hit
GTTCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCC34280.3121281072817448No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC31890.290366550210468No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30800.28044182334532497No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGAACCACTC27090.2466613309878199No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26310.2395592328641396No Hit
CTCCTGCACTGGGAGCATCTCCAACATCGGGGCAGGTTATCATGTACACTGGTACCAGCAGCTGCCAGGAAAAGC26070.23737397190300719No Hit
TCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTTT25110.2286329280584776No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24850.22626556201725082No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24340.22162188247484446No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24270.22098451469451416No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23160.2108776827492768No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22710.20678031844715358No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21730.19785716952252957No Hit
GATTATTACTGCCAGTCCTTTGACAGCAGGCTGAGTGGTTCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTC21570.19640032888177464No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21540.1961271712616331No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20960.19084612393889647No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20440.18611139185644293No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19940.18155876485408376No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19750.17982876659318728No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19590.17837192595243231No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT18900.17208930068917666No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18880.1719071956090823No Hit
ATGGTAACACCAATCGTCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGG18870.17181614306903512No Hit
CATGTACACTGGTACCAGCAGCTGCCAGGAAAAGCCCCCAAACTCCTCAT18800.17117877528870484No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18470.1681740414671478No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGTGATGGCCAGGGAGG18350.1670814109865816No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18050.16434983478516607No Hit
GGACAGAGGGTCATCATCTCCTGCACTGGGAGCATCTCCAACATCGGGGCAGGTTATCATGTACACTGGTACCAG17930.16325720430459986No Hit
GTCCAGTGATGGCCAGGGAGGCTGAGGAGCCAGACTTGGAGCCAGAGAAT16830.1532414248994097No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTTTGACAGCAGGCTGAGTGGTT16800.15296826727926816No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16240.1478693250366259No Hit
ATCATGTACACTGGTACCAGCAGCTGCCAGGAAAAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGTCCCT16230.1477782724965787No Hit
CCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCATCTCCAACATC16050.1461393267757294No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16030.14595722169563505No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15950.14522880137525757No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15860.14440932851483293No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTTTTCCTGGCAGCTGCTGGTA15320.139492491352285No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGAACCACTCA15280.13912828119209628No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15220.13858196595181316No Hit
GCCTCATCCTCAGCCTGGAGTCCAGTGATGGCCAGGGAGGCTGAGGAGCC14950.13612354737053922No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT14800.13475775926983147No Hit
GGTTATCATGTACACTGGTACCAGCAGCTGCCAGGAAAAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGT14720.134029338949454No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14460.13166197290822723No Hit
GTCAAAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAG14210.12938565940704766No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13610.12392250700421663No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13540.12328513922388637No Hit
GGCCATCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTTTGACAGCAGGCTGAGTGGTT13400.12201040366322578No Hit
CACCAATCGTCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGCCATCA13270.1208267206426124No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12910.11754882920091379No Hit
GTGATGGCCAGGGAGGCTGAGGAGCCAGACTTGGAGCCAGAGAATCGGTC12690.11554567331987575No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC12560.11436199029926239No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGCCATCACTGGACTCCAGG12480.11363356997888491No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCATCATCTCCTG12470.11354251743883773No Hit
CCCCTGAGGGACGATTGGTGTTACCATAGATGAGGAGTTTGGGGGCTTTTCCTGGCAGCTGCTGGTACCAGTGTA12400.11290514965850745No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12390.11281409711846027No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12070.1099004158369504No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11990.10917199551657294No Hit
TGGTAACACCAATCGTCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGC11960.10889883789643139No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11930.10862568027628983No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11870.10807936503600672No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11860.10798831249595954No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGAACCA11730.10680462947534616No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11720.10671357693529898No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC11680.10634936677511024No Hit
GTGTCTGGGGCCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCAT11640.10598515661492151No Hit
ACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTTTGACAGCAGGCTGAGTGGTTCTTGGGTGTTCGG11490.10461936851421376No Hit
CTCCAACATCGGGGCAGGTTATCATGTACACTGGTACCAGCAGCTGCCAG11410.10389094819383629No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11410.10389094819383629No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11360.10343568549360037No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11340.10325358041350599No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11340.10325358041350599No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG11090.1009772669123264No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11060.10070410929218487No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11030.10043095167204333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)