FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098267
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43600.3969890746057197No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCA39640.3609322687470351No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38050.34645491487953295No Hit
GTTCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCC36220.3297923000508984No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCATCATCTCCTG35050.3191391528653779No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33230.30256759057679056No Hit
CCCAAGAACCACTCAGCCTGCTGTCAAAGGACTGGCAGTAATAATCAGCC30590.2785297200043341No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29120.2651449966173981No Hit
CTCCTGCACTGGGAGCATCTCCAACATCGGGGCAGGTTATCATGTACACTGGTACCAGCAGCTGCCAGGAAAAGC26680.2429281768458854No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26220.23873976000371497No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC25870.23555292110206352No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25520.2323660822004121No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25510.23227502966036492No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25220.2296345059989966No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24860.22635661455729802No Hit
TCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTTT22470.20459505748602114No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22350.20350242700545498No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21750.19803927460262397No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21620.19685559158201058No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21420.19503454078106688No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGAACCACTC21380.19467033062087816No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20800.18938928329814153No Hit
GATTATTACTGCCAGTCCTTTGACAGCAGGCTGAGTGGTTCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTC20320.18501876137587672No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT19430.1769150853116774No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19300.17573140229106401No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18880.1719071956090823No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCAT18600.16935772448776118No Hit
CATGTACACTGGTACCAGCAGCTGCCAGGAAAAGCCCCCAAACTCCTCAT18470.1681740414671478No Hit
ATGGTAACACCAATCGTCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGG18470.1681740414671478No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCAT18210.165806675425921No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18070.16453193986526046No Hit
GGACAGAGGGTCATCATCTCCTGCACTGGGAGCATCTCCAACATCGGGGCAGGTTATCATGTACACTGGTACCAG17810.16216457382403368No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17570.1599793128629013No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17490.1592508925425238No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16630.15142037409846604No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT16320.14859774535700337No Hit
ATCATGTACACTGGTACCAGCAGCTGCCAGGAAAAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGTCCCT16220.14768721995653153No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15560.14167775231341742No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15450.1406761743728984No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTTTGACAGCAGGCTGAGTGGTT15310.13940143881223782No Hit
GGTTATCATGTACACTGGTACCAGCAGCTGCCAGGAAAAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGT15310.13940143881223782No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15300.13931038627219064No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15270.1390372286520491No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14600.1329367084688878No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14080.12820197638643427No Hit
GTCCAGTGATGGCCAGGGAGGCTGAGGAGCCAGACTTGGAGCCAGAGAAT14080.12820197638643427No Hit
CCCCAGGACAGAGGGTCATCATCTCCTGCACTGGGAGCATCTCCAACATC14040.12783776622624554No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTTTTCCTGGCAGCTGCTGGTA13900.12656303066558497No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13850.12610776796534903No Hit
CTCCAACATCGGGGCAGGTTATCATGTACACTGGTACCAGCAGCTGCCAG13710.12483303240468847No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT13510.12301198160374481No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGTGATGGCCAGGGAGG13390.1219193511231786No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13230.12046251048242367No Hit
GGCCATCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTTTGACAGCAGGCTGAGTGGTT13190.12009830032223494No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13170.11991619524214056No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13040.11873251222152718No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGAC13020.11855040714143282No Hit
CACCAATCGTCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGCCATCA12600.11472620045945112No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC12480.11363356997888491No Hit
TGGTAACACCAATCGTCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGC12060.1098093632969032No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11950.1088077853563842No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11700.10653147185520462No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11610.10571199899477995No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGCCATCACTGGACTCCAGG11510.10480147359430814No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC11390.10370884311374191No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11360.10343568549360037No Hit
GTCAAAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAG11270.10261621263317573No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11240.10234305501303417No Hit
GCCTCATCCTCAGCCTGGAGTCCAGTGATGGCCAGGGAGGCTGAGGAGCC11200.10197884485284543No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11160.1016146346926567No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11060.10070410929218487No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGAACCA10990.10006674151185459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3150.045.5409851
GGTATCA11500.042.471921
GTATCAA29650.039.3971751
AATACGC350.001419702839.0137985
TGGTATC4200.036.58212
TATCAAC34400.033.7462772
ATCAACG34550.033.3945163
TCAACGC35100.032.871244
CAACGCA36050.032.1011625
AACGCAG36250.032.018226
TCTGGAG4850.031.08965770
ATAAACG553.466193E-431.0322913
CGCAGAG37850.030.7549328
ACGCAGA38350.030.442977
AGAGTAC39300.029.70707111
GCAGAGT39750.029.1990079
CAGAGTA39950.029.13827910
CGATTAA1000.006365535328.7209270
AATGGTA3850.027.97492270
CAATACG500.0081461527.3084144
Produced by FastQC (version 0.11.5)