FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779772
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT33230.42615020800952075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26160.33548267955248456No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25970.33304606987683577No Hit
GTCATTATACTGCTGACAATAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT22070.2830314502187819No Hit
GTATATTACTGTGCGGGAGACTCCGGTGGATTCAGGTCCAAGAGTTACTA20860.2675140938633344No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19290.24738000338560504No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGTCGCTCATTTGCAGATCCA18540.23776180729751772No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16200.2077530355026854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16190.20762479288817756No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15450.1981348394145981No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTATTGTCAGCAGTATAATGACTGG14260.1828739682881663No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14230.1824892404446428No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14200.18210451260111932No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCAGTGGCCCTGGC14180.18184802737210365No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13540.17364050004360249No Hit
CTCCTGCAGGGCCAGTCAGAATCTTAACAGCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT13380.1715886182114772No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12970.16633067101665613No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12870.16504824487157785No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATCTTAACAG12240.15696896015758452No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12140.1556865340125062No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAC11960.15337816695136527No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11340.14542712485187978No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11320.1451706396228641No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11320.1451706396228641No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATCTTAACAGCAACTTAGCCTGGTA11290.14478591177934064No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11270.14452942655032497No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11090.142221059489184No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10970.14068214811509006No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10960.14055390550058222No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10770.13811729582493346No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10520.13491123046223769No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10440.13388528954617504No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10270.13170516509954192No Hit
CATCTATCGTGCGTCCGCCAGGGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA10030.12862734235135398No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9630.12349763777104077No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9630.12349763777104077No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9510.12195872639694681No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCA9490.12170224116793114No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATCGTGCGTCCGCCAGGGCCACTGGTGTCCCAGACAGGTT9340.11977860195031369No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9240.11849617580523537No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9230.11836793319072755No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9130.11708550704564924No Hit
GTCTGGGACACCAGTGGCCCTGGCGGACGCACGATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTA9050.1160595661295866No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG8690.1114428320073047No Hit
CCAGTGGCCCTGGCGGACGCACGATAGATGAGGAGCCTGGGAGCCTGGCC8470.10862149448813242No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8310.10656961265600713No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8220.10541542912543667No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8180.10490245866740534No Hit
GGTTTGTGCTGGTACCAGGCTAAGTTGCTGTTAAGATTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC8150.10451773082388185No Hit
GGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT8120.10413300298035837No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTC8080.10362003252232703No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8030.1029788194497879No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC8030.1029788194497879No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8030.1029788194497879No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7940.10182463591921741No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7920.10156815069020175No Hit
CCTCATCTATCGTGCGTCCGCCAGGGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT7850.10067045238864693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTT100.006995432145.6458770
GCGTATG501.1386874E-955.07148440
TCGTATG1350.050.9921240
CGTATGC1650.050.13379341
TCTGGAG6350.048.16635570
GCCGTCT1600.047.8019246
CGTATGG306.3399604E-445.9559841
CGTCTTC1550.045.0693448
ATCTCGT1150.044.63850837
GGTATCA7900.044.041571
ATGCCGT2050.043.94140644
GGCGTAT551.5270962E-743.69264239
GAGCGAT3350.043.6526932
ACGAGAC3900.043.31810822
GATCTCG1200.042.72581536
TCTCCGA4150.041.50145711
GTCGTAT503.9738297E-641.1959239
CGAGCCC4200.041.0126515
CGAGACA4300.039.2960823
CACGAGA4350.038.83194421
Produced by FastQC (version 0.11.5)