FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779772
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38740.4968118886033353No Hit
GTATAATGACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT31340.40191235386754076No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26580.3408688693618135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23800.3052174225286366No Hit
GTCATTATACTGCTGACAATAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT21410.27456743766126507No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19670.2522532227369026No Hit
GTATATTACTGTGCGGGAGACTCCGGTGGATTCAGGTCCAAGAGTTACTA19070.24455866586643277No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGTCGCTCATTTGCAGATCCA16590.2127544974684908No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16250.20839424857522454No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15010.19249216437625358No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14970.19197919391822227No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14770.18941434162806564No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTATTGTCAGCAGTATAATGACTGG14690.188388400712003No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT14670.18813191548298733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14450.18531057796381506No Hit
CTCCTGCAGGGCCAGTCAGAATCTTAACAGCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT13500.17312752958557118No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12490.1601750255202803No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11900.1526087112643183No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCAGTGGCCCTGGC11770.15094155727571648No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11670.1496591311306382No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAC11340.14542712485187978No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11200.14363172824877016No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATCTTAACAGCAACTTAGCCTGGTA11170.14324700040524666No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATCTTAACAG11060.14183633164566053No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10910.13991269242804308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10510.13478298784772985No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10490.13452650261871418No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10440.13388528954617504No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10200.1308074667979871No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10020.12849909973684617No Hit
CATCTATCGTGCGTCCGCCAGGGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA9970.127857886664307No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9970.127857886664307No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9870.1265754605192287No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATCGTGCGTCCGCCAGGGCCACTGGTGTCCCAGACAGGTT9720.12465182130161125No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9500.12183048378243896No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9450.12118927070989981No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCA9260.11875266103425104No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9220.1182396905762197No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9140.11721374966015707No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8970.11503362521352396No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8790.112725258152383No Hit
GGTTTGTGCTGGTACCAGGCTAAGTTGCTGTTAAGATTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC8640.11080161893476555No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8560.1097756780187029No Hit
GTCTGGGACACCAGTGGCCCTGGCGGACGCACGATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTA8340.10695434049953062No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8180.10490245866740534No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8110.10400476036585052No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8070.10349178990781921No Hit
CCTCATCTATCGTGCGTCCGCCAGGGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT8040.10310706206429572No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8000.10259409160626438No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8000.10259409160626438No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC7930.10169639330470959No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTC7890.10118342284667826No Hit
CCAGTGGCCCTGGCGGACGCACGATAGATGAGGAGCCTGGGAGCCTGGCC7880.10105518023217043No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA7860.10079869500315478No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7820.10028572454512344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)