FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167286
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATCCTCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT47770.40923989493577406No Hit
CTATTATACTGTTGGCAGTAATAAGTTACAAAATCTTCAGGCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTC44860.3843102718613947No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29400.25186629497826585No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26110.22368125720688845No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24820.21262998099865843No Hit
GATCTATGGTGCATCCAGTTTGCAGAATGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA24780.2122873057673955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21640.18538730011325416No Hit
AGTCAGGACATTACCAATTATTTAGGTTGGTTTCAGCAGGAACCAGGGAA18760.160714683462322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17260.1478643622899615No Hit
CAATAATACACAGCCGTGTCCTCGGTTCTCAGGCTGTTCATTTGCAGATA17170.14709334301961988No Hit
CCATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTCCTGCTGAAACCAACC16340.13998283197091374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15850.13578506038794264No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15020.1286745493392365No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14550.12464811537189685No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14530.1244767777562654No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13880.11890830524824249No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC13880.11890830524824249No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT13660.1170235914762963No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13500.11565289055124452No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13440.11513887770435009No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTACCAATTATTTAGGTTGGTTTCAGCAGGAA13370.11453919604963994No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13260.11359683916366684No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13040.11171212539172062No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12620.10811403546345968No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12510.10717167857748659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT252.6043993E-455.039439
ACGAAAC200.00783750650.93199528
GAACCGT200.00793515350.7727176
GGTATCA14450.048.022391
CTAACGC1000.047.3878673
CGTATGC3000.047.18744741
TCGTATG2800.046.77072540
TGCCGTC2650.045.92937545
GCCGTCT2450.045.5006446
TATCTCG2250.044.1240636
ATCTCGT2350.043.75629837
CCGTCTT2650.042.14467247
ATGCCGT3250.040.5779644
GACCGGA4050.039.34499726
CACGAGA4250.039.0386821
AGACCGG4100.038.85017825
GTATGCC3750.038.74857342
CTTATAC4400.038.4658241
CGAGCCC4600.038.26185615
ACGAGAC4250.038.2436122
Produced by FastQC (version 0.11.5)