FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167286
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATCCTCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT46230.3960468985321507No Hit
CTATTATACTGTTGGCAGTAATAAGTTACAAAATCTTCAGGCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTC43130.36948956810927225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41500.3555255524353072No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26960.23096310587122607No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24420.20920322868602897No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22810.19541055062769533No Hit
GATCTATGGTGCATCCAGTTTGCAGAATGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA22320.19121277904472425No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22000.1884713771946207No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16730.14332391547572745No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16230.13904047508494063No Hit
AGTCAGGACATTACCAATTATTTAGGTTGGTTTCAGCAGGAACCAGGGAA15850.13578506038794264No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15690.13441435946289085No Hit
CAATAATACACAGCCGTGTCCTCGGTTCTCAGGCTGTTCATTTGCAGATA15300.1310732759580771No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15280.13090193834244565No Hit
CCATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTCCTGCTGAAACCAACC15250.13064493191899842No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14230.12190671352179329No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14180.1214783694827146No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14170.12139270067489885No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12940.11085543731356325No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12780.10948473638851147No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12350.1058009776524348No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC12320.10554397122898758No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12260.10502995838209317No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTACCAATTATTTAGGTTGGTTTCAGCAGGAA12130.1039162638804886No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12100.10365925745704138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11950.10237422533980532No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11740.10057518037567485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)