FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749172
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCCGTGCTCCTACCTGCATATGAGCAG30490.40698264217028934No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27210.3632009738751582No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCCGTGCTCCTACCTGCATATGAGCAGCAGTAATAGT25700.34304538877587526No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24960.33316781727026634No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT22470.29993112396085275No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21560.2877843806228743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20990.2801759809496351No Hit
GACTATTACTGCTGCTCATATGCAGGTAGGAGCACGGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20870.2785742125973742No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG19950.26629398856337394No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTCCAGATACA18660.24907497877656934No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18370.24520403859193884No Hit
CTATTACTGCTGCTCATATGCAGGTAGGAGCACGGAGGTGTTCGGCGGAG18180.24266790536752575No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC17410.23238989177385166No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17280.23065464272556904No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17270.23052116202954726No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17120.22851895158922117No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16440.21944226425974275No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAACAGCA15980.21330215224274265No Hit
GTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG15740.21009861553822087No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15320.20449242630530773No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15310.20435894560928597No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15170.20249021586498162No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14680.1959496617599163No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14630.19528225827980758No Hit
GCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG14420.19247916366335102No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13840.18473728329409No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13790.18406987981398132No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13650.18220115006967694No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13500.1801989396293508No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13480.17993197823730733No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13310.17766280640493773No Hit
GTGTATTACTGTGCAAGAGGCGGAACGGGAACCCCTCGCCCTTTTGACTA12820.17112225229987238No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12790.17072181021180718No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12550.16751827350728538No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12250.16351385262663315No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11940.15937595104995916No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11500.15350280042500253No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11280.1505662251125242No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11250.15016578302445902No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCCGTGCTCCTAC10720.1430913061353067No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10600.14148953778304582No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10360.138286001078524No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10110.1349489836779805No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9850.13147848558141523No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9730.1298767172291543No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9530.12720710330871948No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9440.12600577704452381No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC9270.12373660521215422No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9120.12173439477182808No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9040.12066654920365417No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8900.1187978194593498No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8800.11746301249913238No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8600.11479339857869755No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAGCTCATGATTTATGGGGTCA8520.11372555301052362No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC8480.11319163022643665No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8470.11305814953041492No Hit
GATCAGGACTGCACACAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGG8420.1123907460503062No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8250.1101215742179366No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8120.10838632516965398No Hit
CCTCCGTGCTCCTACCTGCATATGAGCAGCAGTAATAGTCAGCCTCGTCC8100.10811936377761047No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTACTGCTGCTCATAT7990.10665107612137133No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7850.10478234637706695No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7680.10251317454469736No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7600.10144532897652342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.056.24290541
GTCGTAT252.6059136E-455.0303439
TCTGGAG5650.053.88593370
TCGTATG1500.052.81221840
TCATCGG200.00689214652.61303351
CGGCATT200.007198984752.0367245
CTCGCTA200.007764876451.0503123
CACGAGA1850.049.6705721
CTTATAC1950.047.1265071
GTATGCC1700.046.76644542
ACGAGAC2050.046.48483322
GAGACCG2000.045.9422124
TGTCGTA306.4363104E-445.8153538
TATACAC2050.044.824663
GATCTCG1300.044.81348836
CGAGACC2100.043.7574123
TCCGAGC2100.043.75448613
GGTATCA6000.043.679291
ATGCCGT1750.043.57022544
AGACCGA2050.043.1673725
Produced by FastQC (version 0.11.5)