FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293188
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT52200.40365360643618714No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC44750.3460440400003712No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44570.3446521310126602No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40810.3155766988249195No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCACTACCCGCATATGAGCAGCAGTGATAAT40770.3152673857165393No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38250.2957806598885854No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGGCA36810.28464538798689754No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35190.27211820709749857No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34470.2665505711466546No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC34430.26624125803827436No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCACTAC34020.26307079867737715No Hit
CTCCTGGACAGTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGATGTT33630.26005499587067No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32270.24953835018574252No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCCTG30820.23832575000695952No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG29800.23043826574326393No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCACTACCCGCATATGAGCAG29050.2246386449611348No Hit
GTGTTGGTACCAGGAGACAAGGTTAAACCTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGCGATCGA28620.22131352904604745No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGGTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC27640.21373535789073203No Hit
GATTATCACTGCTGCTCATATGCGGGTAGTGACACCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT27330.21133818130078535No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23590.18241740566723477No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23020.17800969387281662No Hit
ATAATATACAGCCGTGTCCTCGGCTCTCAAATTGTCCATTTGAAGATACA22630.1749938910661095No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22620.17491656278901443No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22600.17476190623482432No Hit
GTGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG22520.17414328001806387No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCA22060.17058617927169134No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21730.16803434612755455No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20990.16231205362252046No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCT20480.15836831149067268No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20020.15481121074430013No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC19790.15303266037111385No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19630.151795407937593No Hit
GATCTGCACTGAGCACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG18220.14089212086719022No Hit
GTATATTATTGTGCGAGATTCAGTTATGGTTCGTTTGACTTCTGGGGCCA18170.14050547948171496No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC17080.13207669727835397No Hit
GTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAAC17030.1316900558928787No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17020.13161272761578363No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17000.13145807106159352No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16960.13114875795321332No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA16330.12627707649622483No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15850.1225653191956622No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15810.12225600608728197No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGACAAGGTTAAAC15610.12070944054538087No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15570.12040012743700064No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15170.11730699635319845No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15140.1170750115219133No Hit
GGTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA14970.11576043081129735No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14940.11552844598001219No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14800.11444585010068142No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14690.11359523905263581No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14560.1125899714504001No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14430.11158470384816438No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA13780.10655836583698582No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13730.10617172445151052No Hit
CTCCTGCACAGCCTCTGGATTCACCGTCAGTAGCAATTATATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGG13540.10470248718670448No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13510.10447050235541933No Hit
AGGTTAAACCTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGCGAT13510.10447050235541933No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC13170.10184134093418745No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13000.10052676022357152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)