FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293188
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45980.35555541808306296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA41190.3185151733545316No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40570.3137208201746382No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCACTACCCGCATATGAGCAGCAGTGATAAT39770.3075345580070338No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC38970.30134829583942935No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37490.2899037108293612No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36900.285341342480753No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36050.27876843892767333No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGGCA35610.2753659947354909No Hit
CTCCTGGACAGTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGATGTT34380.26585461665279914No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33960.26260682901480686No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCCTG32770.2534047640404953No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30270.23407269476673154No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCACTACCCGCATATGAGCAG29740.2299742960806936No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCACTAC29520.2282730739846024No Hit
GTGTTGGTACCAGGAGACAAGGTTAAACCTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGCGATCGA28560.22084955938347714No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGGTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC28040.21682848897453424No Hit
GATTATCACTGCTGCTCATATGCGGGTAGTGACACCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT27160.21002360059016942No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26370.20391466669966007No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25320.19579519760467928No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24770.19154214236445125No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23300.18017488563147818No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGGTGTCA22750.17592183039125017No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC22030.1703541944404062No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21810.16865297234431498No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21450.165869154368893No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20580.15914159426162322No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19360.1497075444560265No Hit
ATAATATACAGCCGTGTCCTCGGCTCTCAAATTGTCCATTTGAAGATACA19240.14877960513088584No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCT18960.1466144133722243No Hit
GTGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG18920.14630510026384408No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18340.14182006019233087No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18120.14011883809623968No Hit
GTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAAC17860.13810830289176823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17670.1366390656269622No Hit
GATCTGCACTGAGCACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG17440.13486051525377596No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17010.13153539933868857No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC16360.12650906132751No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16350.12643173305041494No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA16220.12542646544817923No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGACAAGGTTAAAC16170.12503982406270395No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16110.12457585440013362No Hit
GTATATTATTGTGCGAGATTCAGTTATGGTTCGTTTGACTTCTGGGGCCA16040.12403455646046824No Hit
GGTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA15740.12171470814761659No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15390.11900821844928967No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15300.11831226395543418No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15120.11692035496772318No Hit
CTCCTGCACAGCCTCTGGATTCACCGTCAGTAGCAATTATATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGG14480.11197134523363965No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14180.109651496920788No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC14070.10880088587274239No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13930.10771828999341163No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13790.10663569411408086No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA13580.10501180029508471No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13580.10501180029508471No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13540.10470248718670448No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13510.10447050235541933No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13260.10253729542804295No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13230.10230531059675776No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12970.10029477539228634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.002310191769.33955445
CTCGACG150.002502422367.955874
CCTAACG353.6528218E-758.247892
ATCGGAC252.4328727E-455.80415749
CGACTCG200.00714258452.14251747
TCGTATC200.007177287752.07864446
CTAACGC409.1782385E-750.9708443
AATGGTA4600.043.26712870
GGTATCA12450.041.7737921
TCTGGAG10150.037.7649970
GTGGTAT3800.037.5705451
TAACGCC558.210523E-637.066844
TGGTATC4050.036.0753442
GTATCAA29800.033.99241
TCAACGG400.002797474233.9792485
TATCAAC31500.031.1734072
TAGGGAC901.9114123E-730.81758145
ATCAACG33200.029.681823
TCAACGC33150.029.4167984
CATTCGT500.006033089529.04107965
Produced by FastQC (version 0.11.5)