FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185154
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGGCAGCTCACCCTACACTTTTGGCCAGGGGA45620.384928878441114No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34160.28823258411986963No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGGCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33050.2788667126803774No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGTGACGGCTACTTAGCCTGGTCCC25720.2170182102916583No Hit
GTGTATTACTGTGCGACAGATTCGCGCCGATCTTGGGTCTGGGGCCAAGG24740.20874924271444892No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23820.2009865384582932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23430.19769582687144457No Hit
GTCTGGGGCTGAGGTGAAGAAGTCTGGGTCCTCGGTGAAGGTCTCCTGTA23140.19524888748635197No Hit
GTGTATTACTGTCACCAGTATGGCAGCTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAA22900.19322383420213746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22420.18917372763370835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21830.18419547164334762No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19850.16748878204857764No Hit
GTCTGGGACAGACTTCACTCTCACCATCGCCAGACTGGAGCCTGAGGATT19830.16732002760822645No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCCTCTCAGCTCCATGTAGA19810.16715127316787523No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19720.16639187818629478No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18880.1593041916915439No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGGCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18630.15719476118715373No Hit
GTCTACATGGAGCTGAGAGGCCTGAGATCTGAGGACACGGCCGTGTATTA18400.1552540851231148No Hit
GTCCCAGACCCACTGCCACTGAATCTGTCTGGGATGCCAGCGGCCCTGGT17570.14825077584853952No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17430.14706949476608103No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17290.14588821368362254No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16830.14200686155554468No Hit
GTATAACAGACTACGCACAGAAGTTTCAGGGCAGAGTCACGATTACCGCG16740.14124746657396423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16030.1352566839414962No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGTGA15830.1335691395379841No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15580.13145970903359394No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG15050.12698771636428682No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14960.12622832138270637No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT14840.12521579474059913No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14670.12378138199761382No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14600.12319074145638456No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14540.12268447813533094No Hit
GTATGGCAGCTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCAT14500.12234696925462851No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14430.12175632871339927No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14160.11947814376865791No Hit
GTGTAGGGTGAGCTGCCATACTGGTGACAGTAATACACTGCAAAATCCTC13030.10994351788881444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13030.10994351788881444No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12890.10876223680635598No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12810.10808721904495112No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12810.10808721904495112No Hit
GTATTACTGTGCGACAGATTCGCGCCGATCTTGGGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATC12760.10766533294407309No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG12670.10690593796249263No Hit
CATCTATGCCGCATCCACCAGGGCCGCTGGCATCCCAGACAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCA12660.10682156074231704No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12550.10589341132038538No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12500.10547152521950734No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12330.10403711247652203No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGGGTGAG12200.10294020861423916No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12150.10251832251336113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.047.399251
GTATCAA29200.037.53741
AACTGTG20700.037.051170
TATCAAC31750.034.0765652
TCAACGC33650.032.4471744
CAACGCA33950.032.3614585
ATCAACG33650.032.343053
AACGCAG34400.032.037316
GGTACGT752.7713191E-531.03101368
TCAGCAT14200.030.9336470
TTAACGG1001.5150363E-830.91039835
TAACGGC902.0679363E-730.54176136
ACGCAGA36250.030.40237
ATGGCAA3900.030.3922770
GAGCGGT450.00487991530.32909211
CGCAGAG36350.030.3186658
AGAGTAC37000.029.60160811
GTACGTA600.00571336429.36576869
CGGTACC1103.477726E-828.37883645
GAGTTTA3700.027.6860831
Produced by FastQC (version 0.11.5)