FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524555
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAACACTGATGGCACCCACAAGAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGA36520.6962091677707771No Hit
GCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGG31080.5925022161641772No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31060.5921209406068001No Hit
GTGTTAAGGTTCATCAAGAATCGAGGTCCCGTCTCTGGCCGCTGCTGATGCCATGCGATGCCGTTGGTGCTGTGC26500.5051901135247971No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25720.4903203667870862No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25210.4805978400739675No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTC19950.38032236848376244No Hit
GACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGACCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCT19800.3774628018034334No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGATGAGGTAGT17570.33495057715587495No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17370.3311378215821029No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16090.30673618590996177No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15740.3000638636558607No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15380.293200903623071No Hit
GACTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCGGCGGAGGGAC15290.2914851636148736No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14910.2842409280247067No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14740.2810000857870004No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14280.27223074796732466No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTCTT13540.25812355234436807No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13240.25240441898371No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13150.25068867897551256No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13030.24840102563124933No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12910.2461133722869861No Hit
CTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGG12900.24592273450829752No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT12870.24535082117223167No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12730.24268189227059128No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12680.24172870337714822No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12630.2407755144837052No Hit
CCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT12550.2392504122541964No Hit
GGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCAC12530.2388691366968192No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12520.23867849891813062No Hit
CCTCCTCCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGC12480.2379159478033762No Hit
GTTTGGTCCCTCCGCCGAACACGGGACCAGTGCCCCAGGTCTGACAGTAA12470.2377253100246876No Hit
GCTCTGGGGCTGACCACTACCTCATCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC12320.23486574334435853No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGATGAGGTAGTGGTCAGCCCCAGAGCTGGAGC12170.2320061766640295No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTGTCT12130.23124362554927508No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11880.22647768108206004No Hit
CACCTGCACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATC11770.2243806655164854No Hit
GCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGACGGGACCTCGA11680.222664925508288No Hit
CTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCGGCGGAGGGACCA11530.21980535882795893No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11410.2175177054836957No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11290.21523005213943247No Hit
GACCACTACCTCATCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGT10920.20817645432795417No Hit
GCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAC10870.20722326543451117No Hit
CAAGAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCACTACCTCATCATCTCCAG10830.20646071431975674No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10650.2030292343033619No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGAC10380.1978820142787696No Hit
TCCTCAGACTGGAGGCTGGAGATGATGAGGTAGTGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC10270.19578499871319496No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCGGCGGAGGGACCAAACTGAC10220.19483180981975198No Hit
CTACCTCATCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACT10200.19445053426237477No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9740.18568119644269904No Hit
GTAGTGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGT9530.1816778030902384No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9370.17862759863122074No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9040.17233655193449687No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8640.16471104078695276No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGACCAGTGC8470.1614701985492465No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8450.1610889229918693No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC8350.15918254520498326No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8340.15899190742629465No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8280.15784808075416304No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8210.15651361630334282No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGC8130.154988514073834No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8090.1542259629590796No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8080.15403532518039098No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT8070.15384468740170237No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8010.15270086072957076No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7970.15193830961481636No Hit
ACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCG7960.15174767183612775No Hit
GGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCG7940.15136639627875056No Hit
GGACCAAACTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7920.15098512072137335No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7920.15098512072137335No Hit
CTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCACCTGCACT7900.15060384516399614No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7900.15060384516399614No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA7890.15041320738530753No Hit
GTTCAGAGTGCAGGTGAGGTTGACCGAGGCTCCCAGGGAGGCAGAGGCAG7870.15003193182793034No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7770.1481255540410443No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7400.141071956229566No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7330.13973749177874578No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTCTTGTGGGT7280.13878430288530277No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCT7260.1384030273279256No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7220.13764047621317116No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC7090.13516218509021932No Hit
ACCTTAACACTGATGGCACCCACAAGAAGGGGGACGGGATCCCTGATCGC6960.13268389396726749No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6930.1321119806312017No Hit
CTCCAGCTCTGGGGCTGACCACTACCTCATCATCTCCAGCCTCCAGTCTG6890.1313494295164473No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTGT6890.1313494295164473No Hit
GAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGACGGGACCTCGATTCTTGATGAA6860.13077751618038147No Hit
GCCTCATCCTCAGACTGGAGGCTGGAGATGATGAGGTAGTGGTCAGCCCC6820.13001496506562707No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG6680.12734603616398663No Hit
CCTGCACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAG6610.1260115717131664No Hit
CAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGA6570.125249020598412No Hit
CCCTGGGAGCCTCGGTCAACCTCACCTGCACTCTGAACAGTGGGCACAGC6560.1250583828197234No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6510.12410519392628037No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG6390.12181754058201714No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6320.12048307613119692No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6260.11933924945906531No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6180.11781414722955648No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6070.11571713166398186No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG5880.1120950138688984No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT5870.1119043760902098No Hit
CTGTTCAGAGTGCAGGTGAGGTTGACCGAGGCTCCCAGGGAGGCAGAGGC5850.11152310053283258No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC5780.11018863608201236No Hit
ACCTCACCTGCACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGACGGGAC5760.10980736052463518No Hit
ACCTTAACACTGATGGCACCCACAAGAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG5690.10847289607381495No Hit
GTGCAGGTGAGGTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGCGGCTGAGTCAGCACAACTTGGGAGAGA5680.10828225829512635No Hit
GCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCACCTGCACTCTGAACAG5660.10790098273774913No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGT5630.10732906940168334No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG5630.10732906940168334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.10713843162299473No Hit
ACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGACCAGTGCCCC5590.10656651828692892No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGG5540.1056133293934859No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT5520.1052320538361087No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG5470.1042788649426657No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC5430.10351631382791128No Hit
CTCTGGGGCTGACCACTACCTCATCATCTCCAGCCTCCAGTCTGAGGATG5400.10294440049184547No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA5370.10237248715577965No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG5370.10237248715577965No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT5330.10160993604102525No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5320.10141929826233664No Hit
GATGAGGTAGTGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGA5300.10103802270495943No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG5280.10065674714758224No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA5270.10046610936889364No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC5260.10027547159020503No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT5250.10008483381151642No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG5250.10008483381151642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)