FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524555
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76791.4639075025497803No Hit
CCTTAACACTGATGGCACCCACAAGAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGA32480.6191915051805816No Hit
GTGTTAAGGTTCATCAAGAATCGAGGTCCCGTCTCTGGCCGCTGCTGATGCCATGCGATGCCGTTGGTGCTGTGC28500.5433176692625177No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28370.540839378139566No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26390.5030930979592226No Hit
GCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGG25950.49470503569692403No Hit
GGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCAC25070.47792891117232705No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22490.42874436427066753No Hit
GACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGACCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCT18700.3564926461476871No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTC18660.3557300950329327No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17340.33056590824603715No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16650.31741190151652354No Hit
CCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT16350.31169276815586544No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16060.30616427257389595No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15630.29796684809028606No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15130.28843495915585593No Hit
GACTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCGGCGGAGGGAC14730.2808094480083118No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGATGAGGTAGT14450.2754715902050309No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14340.2733745746394563No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13500.2573610012296137No Hit
CCTCCTCCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGC13430.25602653677879345No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13150.25068867897551256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13100.24973549008206955No Hit
GCTCTGGGGCTGACCACTACCTCATCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC12900.24592273450829752No Hit
CTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGG12880.2455414589509203No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12450.2373440344673104No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTGTCT12390.23620020779517875No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12320.23486574334435853No Hit
CTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCGGCGGAGGGACCA12150.2316249011066523No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12140.2314342633279637No Hit
GCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAC12030.22933724776238906No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11900.22685895663943725No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11800.22495257885255124No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11720.22342747662304238No Hit
GTTTGGTCCCTCCGCCGAACACGGGACCAGTGCCCCAGGTCTGACAGTAA11580.22075854772140194No Hit
CTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCACCTGCACT11040.2104641076722174No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11000.209701556557463No Hit
GACCACTACCTCATCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGT10800.20588880098369094No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTCTT10790.20569816320500234No Hit
GCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGACGGGACCTCGA10780.20550752542631373No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGATGAGGTAGTGGTCAGCCCCAGAGCTGGAGC10750.20493561209024794No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10690.2037917854181163No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCGGCGGAGGGACCAAACTGAC10640.2028385965246733No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10320.19673818760663803No Hit
CAAGAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCACTACCTCATCATCTCCAG9950.1896845897951597No Hit
TCCTCAGACTGGAGGCTGGAGATGATGAGGTAGTGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC9810.18701566089351926No Hit
CACCTGCACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATC9780.18644374755745347No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9770.18625310977876486No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9690.18472800754925603No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9430.17977142530335236No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9170.1748148430574487No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGAC9100.1734803786066285No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8970.17100208748367665No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8870.16909570969679064No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8840.16852379636072481No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGC8810.167951883024659No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC8770.1671893319099046No Hit
GTAGTGGTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGT8750.16680805635252738No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8620.16432976522957554No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8360.15937318298367187No Hit
CTACCTCATCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACT8340.15899190742629465No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8170.15575106518858842No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGACCAGTGC8130.154988514073834No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8040.15327277406563658No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA7920.15098512072137335No Hit
ACCTTAACACTGATGGCACCCACAAGAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG7630.14545662513940388No Hit
GGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGTCCCGTGTTCG7460.14221578290169762No Hit
GGACCAAACTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7420.14145323178694322No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7400.141071956229566No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7310.1393562162213686No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTGTCTCTCCCAAGTTGTGCTGACTCAGCCGCCCTCTGCCTCTGCCT7300.13916557844268No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7250.13821238954923698No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT7190.13706856287710537No Hit
GTGCTGACTCAGCCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCACCTGCACTCTGAACAGTGGG7130.13592473620497375No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7130.13592473620497375No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7100.13535282286890793No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG7070.13478090953284214No Hit
CTCCAGCTCTGGGGCTGACCACTACCTCATCATCTCCAGCCTCCAGTCTG7060.13459027175415353No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7060.13459027175415353No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTGT7050.13439963397546492No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7000.13344644508202191No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6600.1258209339344778No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6530.12448646948365757No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG6510.12410519392628037No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGT6480.12353328059021455No Hit
GTTCAGAGTGCAGGTGAGGTTGACCGAGGCTCCCAGGGAGGCAGAGGCAG6350.12105498946726272No Hit
GAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGACGGGACCTCGATTCTTGATGAA6300.12010180057381971No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6290.1199111627951311No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6270.11952988723775392No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGG6240.1189579739016881No Hit
ACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCG6150.11724223389349069No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6150.11724223389349069No Hit
GCCTCATCCTCAGACTGGAGGCTGGAGATGATGAGGTAGTGGTCAGCCCC6140.11705159611480208No Hit
CCTGCACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAG6040.11514521832791604No Hit
CCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCACCTGCACTCT5830.1111418249754554No Hit
GCCTCTGCCTCCCTGGGAGCCTCGGTCAACCTCACCTGCACTCTGAACAG5810.11076054941807818No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG5730.10923544718856935No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC5670.10809162051643774No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5620.10713843162299473No Hit
CTGTTCAGAGTGCAGGTGAGGTTGACCGAGGCTCCCAGGGAGGCAGAGGC5590.10656651828692892No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC5500.1048507782787315No Hit
ACCTCACCTGCACTCTGAACAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGACGGGAC5490.1046601405000429No Hit
CCCTGGGAGCCTCGGTCAACCTCACCTGCACTCTGAACAGTGGGCACAGC5450.10389758938528848No Hit
ACCTAAGACGGTCAGTTTGGTCCCTCCGCCGAACACGGGACCAGTGCCCC5360.10218184937709106No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5360.10218184937709106No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG5360.10218184937709106No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC5350.10199121159840245No Hit
CAGTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGA5320.10141929826233664No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA5290.10084738492627084No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC5280.10065674714758224No Hit
GTGGGCACAGCACCAACGGCATCGCATGGCATCAGCAGCGGCCAGAGACG5270.10046610936889364No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTCTTGTGGGT5260.10027547159020503No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5260.10027547159020503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)