FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964077
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGCCTGTATGTGAGCCG32560.33773235955219344No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32100.33296095643812684No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGCCTGTATGTGAGCCGCAGTAATAAT28620.2968642546186664No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28000.2904332330301418No Hit
GATTATTACTGCGGCTCACATACAGGCACCAACACTCATGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT26560.27549666676002016No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25900.26865074055288113No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC25860.26823583593426664No Hit
GTAATAATCAGCCTCGTCTTCAGGCTGGAGCCCAGAGATGTTCAGGGAGG23200.24064467879640317No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC23190.24054095264174957No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22880.23732544184748727No Hit
ATCCTGCACTGGGACCAGAGGTGACATTGGTGGTTATGGGTATGTTTCCTGGTACCAACAGCACCCAGGCAAAGC22070.2289236233205439No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTCCATATCCTG21700.2250857555983599No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21220.22010690017498602No Hit
CTCCTGGACAGTCGATCTCCATATCCTGCACTGGGACCAGAGGTGACATT20500.21263861703992526No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17880.18546236452067624No Hit
TCTCTGGGCTCCAGCCTGAAGACGAGGCTGATTATTACTGCGGCTCACAT17570.18224685372641397No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17020.17654191522046475No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16630.1724965951889735No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16350.16959226285867207No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16200.16803637053886775No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15540.1611904443317287No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTC15480.16056808740380696No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15350.15921964739330988No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14710.15258117349547806No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14630.1517513642582491No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14580.15123273348498098No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14450.14988429347448387No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14360.14895075808260128No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14030.14552779497903176No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13930.14449053343249554No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13850.14366072419526657No Hit
GACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTCCATAT13790.14303836726734484No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13260.13754088107070286No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12990.13474027489505505No Hit
CATGAGTGTTGGTGCCTGTATGTGAGCCGCAGTAATAATCAGCCTCGTCT12100.1255086471308827No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGC12030.12478256404830734No Hit
CCATATCCTGCACTGGGACCAGAGGTGACATTGGTGGTTATGGGTATGTT12010.1245751117390001No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11760.12198195787265956No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTC11740.12177450556335231No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11650.1208409701714697No Hit
CCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGCCTGTATGTGAGCCG11540.11969998247027987No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAAACATACCCATAA11480.11907762554235814No Hit
GTTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAA11410.11835154245978279No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC11100.1151360316655205No Hit
GTAATACACAGCCGTGTCTGTGACGCTCACAGAGGTCAGTTTCAGGGAGATCTGGTTCTTGGACGTATCTGGGG10930.11337268703640892No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10880.1128540562631408No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10820.11223169933521908No Hit
GAACATCTCTGGGCTCCAGCCTGAAGACGAGGCTGATTATTACTGCGGCT10790.1119205208712582No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10550.10943109315957127No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10530.10922364085026402No Hit
ACACTCATGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC10510.10901618854095681No Hit
GTGTATTACTGTGCGACAGGACCCGCGGGATATTGTAGTGGTATTGGCTGCAAAAACTACTTTGACTCCTGGGGC10340.10725284391184522No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10310.10694166544788435No Hit
GCCCAGAGATGTTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10200.1058006777466945No Hit
GTGTTGGTGCCTGTATGTGAGCCGCAGTAATAATCAGCCTCGTCTTCAGG10110.1048671423548119No Hit
GTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAACAT10060.1043485115815438No Hit
TCCATATCCTGCACTGGGACCAGAGGTGACATTGGTGGTTATGGGTATGT9860.10227398848847136No Hit
GTCGATCTCCATATCCTGCACTGGGACCAGAGGTGACATTGGTGGTTATGGGTATGTTTCCTGGTACCAACAGCA9820.10185908386985687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)