FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964077
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGCCTGTATGTGAGCCG30330.3146014270644357No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29540.3064070608467996No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28230.29281893458717506No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC26430.2741482267495231No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGCCTGTATGTGAGCCGCAGTAATAAT26160.2713476205738753No Hit
GATTATTACTGCGGCTCACATACAGGCACCAACACTCATGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT25190.261286183572474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24800.25724086354098274No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24590.25506261429325666No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTCCATATCCTG21460.22259632788667297No Hit
ATCCTGCACTGGGACCAGAGGTGACATTGGTGGTTATGGGTATGTTTCCTGGTACCAACAGCACCCAGGCAAAGC21370.22166279249479034No Hit
GTAATAATCAGCCTCGTCTTCAGGCTGGAGCCCAGAGATGTTCAGGGAGG21280.22072925710290772No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC21180.21969199555637153No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20280.21035664163754556No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19650.20382189389436736No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19540.20268090619317752No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19270.1998803000175297No Hit
CTCCTGGACAGTCGATCTCCATATCCTGCACTGGGACCAGAGGTGACATT18770.19469399228484863No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18300.18981886301612838No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTC17990.18660335222186608No Hit
TCTCTGGGCTCCAGCCTGAAGACGAGGCTGATTATTACTGCGGCTCACAT17830.18494373374740813No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16990.1762307367565039No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16040.1663767520644098No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15570.16150162279568955No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15220.15787120738281277No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15140.15704139814558382No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14960.1551743273618186No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14140.1466687826802216No Hit
GACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTCCATAT13910.14428308112318827No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13760.14272718880338395No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13740.14251973649407673No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13480.13982285647308254No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13370.1386818687718927No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13320.1381632379986246No Hit
CCATATCCTGCACTGGGACCAGAGGTGACATTGGTGGTTATGGGTATGTT12840.13318438257525073No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12160.12613100405880442No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12140.12592355174949718No Hit
CATGAGTGTTGGTGCCTGTATGTGAGCCGCAGTAATAATCAGCCTCGTCT12000.12447138558434648No Hit
GTAATACACAGCCGTGTCTGTGACGCTCACAGAGGTCAGTTTCAGGGAGATCTGGTTCTTGGACGTATCTGGGG11940.12384902865642475No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAAACATACCCATAA11760.12198195787265956No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11490.11918135169701174No Hit
CCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGCCTGTATGTGAGCCG11080.11492857935621326No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11070.11482485320155962No Hit
GTTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAA10950.11358013934571617No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10840.11243915164452632No Hit
GTGTATTACTGTGCGACAGGACCCGCGGGATATTGTAGTGGTATTGGCTGCAAAAACTACTTTGACTCCTGGGGC10830.11233542548987269No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10640.11036462855145386No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10630.11026090239680025No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10550.10943109315957127No Hit
GTGTTGGTGCCTGTATGTGAGCCGCAGTAATAATCAGCCTCGTCTTCAGG10340.10725284391184522No Hit
GTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAACAT10290.10673421313857709No Hit
GTCGATCTCCATATCCTGCACTGGGACCAGAGGTGACATTGGTGGTTATGGGTATGTTTCCTGGTACCAACAGCA10290.10673421313857709No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCTC10110.1048671423548119No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10080.10455596389085105No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC10000.10372615465362206No Hit
ACACTCATGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC9840.10206653617916411No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9820.10185908386985687No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC9770.10134045309658876No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9760.10123672694193513No Hit
GAACATCTCTGGGCTCCAGCCTGAAGACGAGGCTGATTATTACTGCGGCT9750.10113300078728152No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9680.10040691770470617No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACATGAGTGTTGGTGC9650.10009573924074529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)