FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685166
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26340.38443238572842203No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACACTCAGGGTGGTGTCATACGACTGG25290.369107632311002No Hit
CTCCTGCACTGGGAGCAACTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCCGGAACAAC25170.36735623192043976No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACACTCAGGGTGGTGTCATACGACTGGCAGTAGTAAT22210.32415502228656995No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21610.3153980203337585No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20770.3031382175998225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16480.24052565363722075No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16310.2380445030839242No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16110.23512550243298705No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15490.2260766004150819No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13820.20170294497975672No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13800.201411044914663No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13520.197324444003351No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13350.19484329345005444No Hit
CCCCCAAACTCCTCATTTATGGTAACATTAATCGGCCCTCTGGGGTCCCT13120.19148644270147672No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13120.19148644270147672No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12920.18856744205053957No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12660.1847727412043213No Hit
CTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCCG11860.1730967386005727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11710.17090748811236983No Hit
GATTACTACTGCCAGTCGTATGACACCACCCTGAGTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT11530.1682803875265264No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11520.16813443749397955No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11490.16769658739633897No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11460.16725873729869842No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11420.16667493716851098No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGCCAGTCGTAT11290.16477758674540186No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11240.16404783658266756No Hit
CCCTGAGTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT10660.15558273469494982No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10560.15412323436948128No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACACTCAGGGTGG10380.15149613378363785No Hit
GTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGCCAGGGAGG10370.15135018375109097No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10350.15105828368599727No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10180.14857713313270068No Hit
CACTCAGGGTGGTGTCATACGACTGGCAGTAGTAATCAGCCTCATCCTCA10020.14624193261195098No Hit
ACACCACCCTGAGTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTA9590.13996608121243612No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAACTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAG9370.13675518049640525No Hit
GATGTACACTGGTACCAGCAGCTTCCCGGAACAACCCCCAAACTCCTCATTTATGGTAACATTAATCGGCCCT9240.1348578300732961No Hit
GGGTGGTGTCATACGACTGGCAGTAGTAATCAGCCTCATCCTCAGCCTGG8750.12770627847850008No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8720.12726842838085953No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT8670.12653867821812523No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8520.12434942772992238No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGCCAGGGAGGCTGAGGTGCC8230.12011687678606352No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACA8230.12011687678606352No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAA8180.11938712662332923No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8130.11865737646059496No Hit
GGATGAGGCTGATTACTACTGCCAGTCGTATGACACCACCCTGAGTGTGGTCTTCGGCGGAGGGACCAAGCTGAC8130.11865737646059496No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7970.11632217593984524No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7900.11530052571201722No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7890.11515457567947039No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7830.11427887548418923No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG7810.11398697541909551No Hit
GTGTCATACGACTGGCAGTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGCCAGGGAGGCTGAGGTG7790.1136950753540018No Hit
GTATGACACCACCCTGAGTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC7730.11281937515872065No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7720.11267342512617382No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7640.11150582486579895No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC7620.11121392480070522No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7600.11092202473561151No Hit
GTTTGGGGGTTGTTCCGGGAAGCTGCTGGTACCAGTGTACATCATAATTT7570.11048417463797094No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7430.10844087418231493No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7430.10844087418231493No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7370.10756517398703379No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCTCTGGGCTCCAGGCTGAGGATG7330.10698137385684638No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7320.1068354238242995No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6930.10114337255497208No Hit
TCCCGGAACAACCCCCAAACTCCTCATTTATGGTAACATTAATCGGCCCTCTGGGGTCCCTGAGCGATTCTCTGG6890.10055957242478465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA200.00679869252.7944451
GGTATCA6700.044.5339431
GTATCAA18300.039.279691
CGTATGC1500.039.0748941
ATGCCGT1450.038.2832244
ATGGCAA2151.0913936E-1137.22489570
CCCACGA1750.036.81011219
TATCAAC19950.035.85582
ATCAACG20650.035.132873
ACGAGAC1750.034.89056822
TAAGTAC400.002673691434.29202737
GTGGTAT1500.033.9065251
TCAACGC21300.033.9015774
ACCAGTA400.002830135233.8966312
CAACGCA21850.033.5136875
AACGCAG21900.033.282376
CTTATAC2050.033.079541
AGACCGA1750.032.9642425
GTGCTAG1350.032.6507261
GTATGCC1750.031.60000442
Produced by FastQC (version 0.11.5)