FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749351
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29770.3972771104595843No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAACAACTCAGGCTGGTGTCATAGGA29150.38900328417523966No Hit
CTCCTGCACTGGGAGCAGCTCCAACCTCGGGGCAAATTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC25480.34002757052436045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24140.3221454298452928No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22160.2957225652598048No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21120.28184388891187173No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAACAACTCAGG20060.267698314941863No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19590.2614262208230856No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16970.22646263233117725No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16820.22446090016560996No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16580.22125812870070236No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15630.20858049165210962No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15570.2077797987858827No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15100.20150770466710524No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14750.19683699628078163No Hit
GATTATTACTGCCAGTCCTATGACACCAGCCTGAGTTGTTGGGTGTTTGG14330.19123214621719328No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14080.18789592594124782No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13640.18202417825558384No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT13250.17681967462510892No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12950.1728162102939744No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12940.17268276148293657No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12910.1722824150498231No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12900.1721489662387853No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12670.16907964358491548No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12620.1684123995297264No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGACAGAGAATCGGTC12600.16814550190765076No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT12070.1610727149226464No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11970.1597382268122682No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAACAACTCAGGC11100.14812818025197805No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACCTCGGGGCAAATTATGATGTACACTGGTACCAG10830.14452506235395696No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG10710.14292367662150315No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10550.14078849564489804No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACACCAGCCTGAGTTGTTGGGTGTTTGGCGGAGGGACCAAG10510.14025470040074678No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10450.13945400753451986No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT10200.13611778725857443No Hit
GTCCTATGACACCAGCCTGAGTTGTTGGGTGTTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10180.1358508896364988No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10000.13344881103781805No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG9960.1329150157936668No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT9920.1323812205495155No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9580.1278439609742297No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9510.126909819296965No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGCTGGTACCAGTGTACATCATAATTT9380.12517498475347333No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG9130.12183876447752788No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC9080.1211715204223388No Hit
GCCCCCAAACTCCTCATTTATGGAAACAACAATCGGCCCTCAGGAGTCCCTGACCGATTCTCTGTCTCCAAGT9030.1205042763671497No Hit
CCTGAGGGCCGATTGTTGTTTCCATAAATGAGGAGTTTGGGGGCTGTTCC9000.12010392993403625No Hit
GCCCTCAGGAGTCCCTGACCGATTCTCTGTCTCCAAGTCTGGCACCTCAG8730.11650081203601517No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8700.11610046560290171No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8630.11516632392563697No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8600.11476597749252354No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8540.11396528462629663No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT8450.11276424532695627No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGAC8210.10956147386204863No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8040.10729284407440573No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8020.10702594645233007No Hit
GTCCCTGACCGATTCTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTGGC8010.10689249764129227No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC7970.10635870239714099No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7950.10609180477506536No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7930.10582490715298973No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC7890.10529111190883844No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG7830.10449041904261154No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7820.10435697023157373No Hit
GCTTGGTCCCTCCGCCAAACACCCAACAACTCAGGCTGGTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCT7770.10368972617638463No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG7650.10208834044393082No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACACCA7600.10142109638874174No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7590.10128764757770391No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7520.10035350590043918No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7500.10008660827836355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)