FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196567
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTGACCTGTACACTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTGCACCAT97530.4440110408651318No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT97450.4436468361766338No Hit
CTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC87620.39889518507744126No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC85140.3876048397340031No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC71950.3275565917178943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62060.2825317871023283No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG60840.27697766560273374No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG57840.2633199897840585No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCTGAAA57630.2623639524767512No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG53930.24551948563371842No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53340.24283347605604566No Hit
GATTTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA51550.23468439615090278No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA50340.22917580023737041No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG48920.22271116701653082No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48400.22034383654129375No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG48260.21970647833642223No Hit
AAAGTGTACAGGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATC46570.21201265429190186No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45710.2080974538905483No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC44380.20204255094426896No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43740.1991289134362849No Hit
GTACACTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTG42290.19252770345725853No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41590.18934091243290097No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41220.1876564657485977No Hit
CCATAAATCAGGAGCTTAGGGGCTTTCCCTGGTTTCAGCTGATACCAATT40770.18560781437579643No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40600.18483387941273813No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTGGGTCCCCCGGCCAAAAGTG39660.18055447432288657No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39450.17959843701557932No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39450.17959843701557932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38430.17495482723722974No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT37410.17031121745888014No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG37230.16949175690975962No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC37040.16862677077457686No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT36960.16826256608607887No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTGGGTCCCCCGGCCAAAAGTGTACAGGTCA36930.1681259893278921No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTTACAGTGAC33350.15182782951760634No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA32550.14818578263262627No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32390.14745737325563027No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31430.14308691699365417No Hit
ACAGTGACCTGTACACTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGA30980.14103826562085292No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30860.1404919585881059No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30790.14017327948567015No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29780.1355751952933828No Hit
CTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTGCACCA29360.13366312067876826No Hit
GGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT29170.1327981345435855No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28670.13052185524047297No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT28640.1303852784822862No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27790.1265156036669949No Hit
GGCCAAAAGTGTACAGGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCA27400.12474010581056713No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA27320.12437590112206912No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC27080.12328328705657511No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27000.1229190823680771No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26130.11895835638066128No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT26010.11841204934791426No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25520.11618129563086399No Hit
GTGTACAGGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATCTTC25210.11477000246293421No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCACCATAAATCAGGAGCTTAGGGGCTTTCCCTGGTTTCAG24940.11354081163925343No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24880.11326765812287992No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24390.11103690440582964No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24100.10971666241002435No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAAC23940.10898825303302835No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC23830.1084874715863436No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23740.10807774131178334No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23700.10789563896753435No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG23470.10684855048810257No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23150.10539173173411055No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT23100.1051641038037993No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22760.10361623387768278No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22720.10343413153343377No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22560.10270572215643775No Hit
AGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCTGAAACCAGGGAA22560.10270572215643775No Hit
GCTTAGGGGCTTTCCCTGGTTTCAGCTGATACCAATTTAAAGAGCTGCTA22560.10270572215643775No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22510.1024780942261265No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22490.102387043054002No Hit
GCTGAAACCAGGGAAAGCCCCTAAGCTCCTGATTTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT22470.10229599188187749No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA22430.10211388953762848No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22290.10147653133275698No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22240.10124890340244572No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG22210.10111232664425898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG2900.036.29459826
GTATCAA55050.035.6644481
CGAGACG3250.032.35496523
TATCAAC60300.031.9918982
TACGTAT2108.6401997E-1031.96380870
CGTAGAG3200.031.86509328
ATCAACG61250.031.6056843
ACGTAGA3350.031.4328227
TTCCGTA450.004473285330.87451645
TCAACGC62650.030.8452974
CGCGCGA450.004632183430.65501641
AACGCAG63100.030.6253226
CAACGCA63550.030.4618115
CCTAACG450.005035267730.136482
CTAACGC450.00503582830.1357943
ACGAGAC3500.029.07010522
AGACGTA3650.028.82431625
AACGGAC500.006732577528.39706456
ACGCAGA68350.028.3206427
CGCAGAG68700.028.1263738
Produced by FastQC (version 0.11.5)