FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196567
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99100.4511585578769052No Hit
GAGTTACAGTGACCTGTACACTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTGCACCAT93250.4245260900304885No Hit
CTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC85950.3912924122050454No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC70750.32209352139042424No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70370.3203635491200587No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC65050.29614393733494127No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG60110.2736542978201894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT56430.2569008821492811No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTCTTTAAATTGGTATCAGCTGAAA54160.24656657411315022No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53970.24570158797796743No Hit
GATTTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA48780.22207380881165928No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA47950.21829518516849247No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG47530.21638311055387793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47030.2141068312507654No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT43610.19853708081747565No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43580.19840050405928888No Hit
AAAGTGTACAGGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATC43340.19730788999379484No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42590.19389347103912605No Hit
GTACACTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTG42270.19243665228513404No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG42100.19166271732207577No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41750.190069321809897No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41530.18906775891652747No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC40990.18660937726916593No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTGGGTCCCCCGGCCAAAAGTG40400.18392336769149312No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38840.176821376265782No Hit
CCATAAATCAGGAGCTTAGGGGCTTTCCCTGGTTTCAGCTGATACCAATT38510.17531903192572776No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37580.17108515242193842No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT37130.16903650104913714No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37030.1685812451885146No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36800.16753415670908287No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT36240.1649847238895968No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTGGGTCCCCCGGCCAAAAGTGTACAGGTCA35890.16339132837741804No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC34900.1588842953572552No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34560.15733642543113868No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT33990.15474146702559038No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAACAGAGTTACAGTGAC33250.15137257365698384No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA32540.14814025704656403No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31380.14285928906334294No Hit
GGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT30970.14099274003479065No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30740.13994565155535887No Hit
CTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTGCACCA29890.13607597674006758No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29280.13329891599027027No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT28810.13115921344534448No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA28390.12924713883072997No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28380.12920161324466772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28130.12806347359311143No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA27960.12728953863005318No Hit
ACAGTGACCTGTACACTTTTGGCCGGGGGACCCAGCTGGAGATCAAACGA27550.1254229896015009No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27380.12464905463844263No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27360.12455800346631812No Hit
GGCCAAAAGTGTACAGGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCA26970.12278250560989035No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT26830.12214514740501883No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC26810.12205409623289433No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA26230.11941361224128379No Hit
GTGTACAGGTCACTGTAACTCTGTTGACAGAGGTAAGTTGCAAAATCTTC25450.11586261652842822No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25100.11426922101624945No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24710.11249372315982166No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24560.1118108393688879No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24510.11158321143857665No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24170.11003534151246012No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC24020.10935245772152637No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23440.10671197372991582No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCACCATAAATCAGGAGCTTAGGGGCTTTCCCTGGTTTCAG23210.10566488525048405No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23210.10566488525048405No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCTCTGTCAAC23010.10475437352923903No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA22860.10407148973830527No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22170.10093022430000997No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22120.10070259636969872No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG22050.10038391726726295No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22030.10029286609513845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT2300.048.3500770
GTGGTAT6500.035.440331
GTATCAA54450.035.4010851
TATCAAC61700.030.8401132
ATCAACG62700.030.6701533
AATGGTA14850.030.42228970
GTACGTA2500.030.41675469
ATGGCAA10350.030.21879670
TCAACGC63100.030.1531164
CAACGCA64200.029.5830675
AACGCAG64600.029.4529676
CGCAGAG68650.027.764718
ACGCAGA68950.027.6439047
AGAGTAC69800.027.45277411
GCAGAGT70350.027.1900239
TGTTAGA28650.026.20016970
GGTACGT2600.025.9178868
TCGACCC5550.025.73602365
TACGAAT951.1742335E-525.06118630
GTGCTAG6550.024.8257371
Produced by FastQC (version 0.11.5)